RNAseq analysis of hippocampal microglia after kainic acid-induced seizures

被引：36

作者：

Bosco, Dale B. ^{[1
]}

Zheng, Jiaying ^{[1
]}

Xu, Zhiyan ^{[2
]}

Peng, Jiyun ^{[1
]}

Eyo, Ukpong B. ^{[1
]}

Tang, Ke ^{[3
]}

Yan, Cheng ^{[3
]}

Huang, Jun ^{[3
]}

Feng, Lijie ^{[4
]}

Wu, Gongxiong ^{[5
]}

Richardson, Jason R. ^{[6
,7
]}

Wang, Hui ^{[2
,8
]}

Wu, Long-Jun ^{[1
,9
]}

机构：

[1] Mayo Clin, Dept Neurol, 200 First St SW, Rochester, MN 55905 USA

[2] Nantong Univ, Sch Pharm, Dept Pharmacol, 19 Qixiu Rd, Nantong 226001, Jiangsu, Peoples R China

[3] Admera Hlth LLC, South Plainfield, NJ 07080 USA

[4] Anhui Med Univ, Sch Basic Med Sci, Dept Histol & Embryol, Hefei 230032, Anhui, Peoples R China

[5] One Harvard St Inst Hlth, Brookline, MA 02446 USA

[6] Northeast Ohio Med Univ, Dept Pharmaceut Sci, Rootstown, OH 44272 USA

[7] Northeast Ohio Med Univ, Ctr Neurodegenerat Dis & Aging, Rootstown, OH 44272 USA

[8] Rutgers Robert Wood Johnson Med Sch, Dept Neurosci & Cell Biol, Piscataway, NJ 08854 USA

[9] Mayo Clin, Dept Neurosci, Jacksonville, FL 32224 USA

来源：

MOLECULAR BRAIN | 2018年 / 11卷

基金：

中国国家自然科学基金;

关键词：

NEUROPATHIC PAIN; INTERFERON-BETA; UP-REGULATION; KAPPA-B; ACTIVATION; CELLS; MOUSE; OSTEOPONTIN; RECEPTORS; SCLEROSIS;

D O I：

10.1186/s13041-018-0376-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Microglia have been shown to be of critical importance to the progression of temporal lobe epilepsy. However, the broad transcriptional changes that these cells undergo following seizure induction is not well understood. As such, we utilized RNAseq analysis upon microglia isolated from the hippocampus to determine expression pattern alterations following kainic acid induced seizure. We determined that microglia undergo dramatic changes to their expression patterns, particularly with regard to mitochondrial activity and metabolism. We also observed that microglia initiate immunological activity, specifically increasing interferon beta responsiveness. Our results provide novel insights into microglia transcriptional regulation following acute seizures and suggest potential therapeutic targets specifically in microglia for the treatment of seizures and epilepsy.

引用

页数：13

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