Chitosan/nano-hydroxyapatite/nano-zirconium dioxide scaffolds with miR-590-5p for bone regeneration

被引：87
作者：
Balagangadharan, K. [1 ]
Chandran, S. Viji [1 ]
Arumugam, B. [1 ]
Saravanan, S. [2 ]
Venkatasubbu, G. Devanand [3 ]
Selvamurugan, N. [1 ]
机构：
[1] SRM Inst Sci & Technol, Sch Bioengn, Dept Biotechnol, Kattankulathur 603203, Tamil Nadu, India
[2] SASTRA Deemed Univ, Sch Chem & Biotechnol, Ctr Nanotechnol & Adv Biomat, Dept Bioengn, Thanjavur 613401, Tamil Nadu, India
[3] SRM Inst Sci & Technol, Sch Bioengn, Dept Nanotechnol, Kattankulathur 603203, Tamil Nadu, India
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 111卷
关键词：
Chitosan; Nano-hydroxyapatite; Nano-zirconium dioxide; miR-590-5p; Bone regeneration; MESENCHYMAL STEM-CELLS; OSTEOBLAST DIFFERENTIATION; IN-VITRO; TISSUE REGENERATION; TARGETING SMAD7; EXPRESSION; RUNX2; PROLIFERATION; MICRORNA-15B; VIVO;
D O I：
10.1016/j.ijbiomac.2018.01.122
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bone tissue engineering (BTE) relies on biocomposite scaffolds and bioactive molecules for bone regeneration. The present study was aimed to synthesize and characterize biocomposite scaffolds containing chitosan (CS), nano-hydroxyapatite (nHAp) and nano zirconium dioxide (nZrO(2)) along with microRNA (miRNA) for BTE applications. miRNAs act as post-transcriptional regulator of gene expression. The fabricated biocomposite scaffolds were characterized using SEM, FT-IR and XRD analyses. The effect of a bioactive molecule (miR-590-5p) with scaffolds was tested for osteoblast differentiation at the cellular and molecular levels using mouse mesenchymal stem cells (C3H10T1/2). The results showed that CS/nHAp/nZrO(2) scaffolds promoted osteoblast differentiation, and this effect was further increased in the presence of miR-590-5p in C3H10T1/2 cells. Thus, we suggested that CS/nHAp/nZrO(2) scaffolds with miR-590-5p would have potential towards the treatment of bone defects. (C) 2018 Elsevier B.V. All rights reserved.
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页码：953 / 958
页数：6
相关论文
共 47 条
[1]   Interaction of stem cells with nano hydroxyapatite-fucoidan bionanocomposites for bone tissue regeneration [J].
Ahn, Tae Young ;
Kang, Jeong Han ;
Kang, Dong Jun ;
Venkatesan, Jayachandran ;
Chang, Hee Kyung ;
Bhatnagar, Ira ;
Chang, Kwan-Young ;
Hwang, Jae-Ho ;
Salameh, Ziad ;
Kim, Se-Kwon ;
Kim, Hui Taek ;
Kim, Dong Gyu .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2016, 93 :1488-1491
[2]  
Amini Ami R., 2012, Critical Reviews in Biomedical Engineering, V40, P363
[3]   Characterization of Runx2 phosphorylation sites required for TGF-1-mediated stimulation of matrix metalloproteinase-13 expression in osteoblastic cells [J].
Arumugam, Balasubramanian ;
Vairamani, Mariappanadar ;
Partridge, Nicola C. ;
Selvamurugan, Nagarajan .
JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (02) :1082-1094
[4]   Chitosan based nanofibers in bone tissue engineering [J].
Balagangadharan, K. ;
Dhivya, S. ;
Selvamurugan, N. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 104 :1372-1382
[5]   Bone regeneration from human mesenchymal stem cells on porous hydroxyapatite-PLGA-collagen bioactive polymer scaffolds [J].
Bhuiyan, Didarul B. ;
Middleton, John C. ;
Tannenbaum, Rina ;
Wick, Timothy M. .
BIO-MEDICAL MATERIALS AND ENGINEERING, 2017, 28 (06) :671-685
[6]  
Chen C.-K., 2017, IMPLANT DENT
[7]   GATA-3 Transduces Survival Signals in Osteoblasts Through Upregulation of bcl-xL Gene Expression [J].
Chen, Ruei-Ming ;
Lin, Yi-Ling ;
Chou, Chih-Wei .
JOURNAL OF BONE AND MINERAL RESEARCH, 2010, 25 (10) :2193-2204
[8]   Zirconium Ions Up-Regulate the BMP/SMAD Signaling Pathway and Promote the Proliferation and Differentiation of Human Osteoblasts [J].
Chen, Yongjuan ;
Roohani-Esfahani, Seyed-Iman ;
Lu, Zufu ;
Zreiqat, Hala ;
Dunstan, Colin R. .
PLOS ONE, 2015, 10 (01)
[9]   Perspectives on RUNX Genes: An Update [J].
Cohen, M. Michael, Jr. .
AMERICAN JOURNAL OF MEDICAL GENETICS PART A, 2009, 149A (12) :2629-2646
[10]  
Della Bella E., 2017, J BIOMED MAT RES A
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