The DNA adsorption by the charged cholesterol monolayer at the air-liquid interface

被引:0
|
作者
Lin, TL [1 ]
Hu, Y [1 ]
Wu, JC [1 ]
Yang, CP [1 ]
Jeng, US [1 ]
Shih, MC [1 ]
机构
[1] Natl Tsing Hua Univ, Dept Engn & Syst Sci, Hsinchu 300, Taiwan
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中图分类号
TQ174 [陶瓷工业]; TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
The adsorption of DNA by the 3-,-[N-(N',N'-dimethyl amino ethane) carbamoyl] cholesterol (DC-Chol) monolayer at the air-liquid interface was studied by using the Langmuir-Blodgett film balance. With the presence of 1 g M DNA in the subphase, the surface pressure increases right at the beginning of the compression. The liquid expanded phase of the DC-Chol disappears due to the adsorption of DNA. The AFM image of the prepared DC-Chol/DNA film has tree-branch-like fractal structure with a height of 2 nm that correspond to the diameter of DNA.
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页码:328 / 329
页数:2
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