Isopentenyl diphosphate isomerase activity in Lactarius mushrooms

被引：2
|
作者：
Ohya, N
Tanaka, Y
Ogura, K
Koyama, T
机构：
[1] TOKYO UNIV AGR & TECHNOL, FAC TECHNOL, KOGANEI, TOKYO 184, JAPAN
[2] TOHOKU UNIV, INST CHEM REACT SCI, AOBA KU, SENDAI, MIYAGI 980, JAPAN
[3] TOHOKU UNIV, FAC ENGN, DEPT BIOCHEM & ENGN, AOBA KU, SENDAI, MIYAGI 98077, JAPAN
来源：
PHYTOCHEMISTRY | 1997年 / 46卷 / 06期
关键词：
Lactarius sp; mushroom; natural rubber; biosynthesis; polyisoprene; isopentenyl diphosphate; isopentenyl diphosphate isomerase;
D O I：
10.1016/S0031-9422(97)00410-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Isopentenyl diphosphate (IDP) isomerase [EC 5.3.3.2] activity, which is presumed to be involved in rubber biosynthesis, was detected in a homogenate of Lactarius mushrooms by measuring the radioactive C-5, alcohols derived from [1-C-14] IDP. Fluoride ions were added to the assay mixture to inhibit phosphatase activity, which interfered with the isomerase activity by hydrolysing the IDP substrate. The level of formation of [1-C-14] DMADP in the homogenate of L. volemus was 41%. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：1115 / 1118
页数：4
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