RETRACTED: MicroRNA-22 functions as a tumor suppressor by targeting SIRT1 in renal cell carcinoma (Retracted article. See vol. 46, 2021)

被引:46
作者
Zhang, Shoulin [1 ]
Zhang, Dongmei [2 ]
Yi, Chunguang [3 ]
Wang, Yinping [1 ]
Wang, Hongan [1 ]
Wang, Jian [1 ]
机构
[1] Changchun Univ Chinese Med, Affiliated Hosp, Dept Internal Med, Changchun 130021, Jilin, Peoples R China
[2] Changchun Univ Chinese Med, Sci Res Off, Affiliated Hosp, Changchun 130021, Jilin, Peoples R China
[3] Changchun Univ Chinese Med, Changchun Jingyue Natl High Tech Ind Dev Zone, Coll Pharm, Changchun 130117, Jilin, Peoples R China
关键词
renal cell carcinoma; miR-22; SIRT1; p53; PANCREATIC-CANCER; MIR-22; PROLIFERATION; EXPRESSION; INVASION; STATISTICS; MIGRATION; P53;
D O I
10.3892/or.2015.4333
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Accumulating evidence demonstrates that microRNA-22 (miR-22) was deregulated in many types of cancers and was involved in various cellular processes related to carcinogenesis. However, the exact roles and mechanisms of miR-22 remain unknown in human renal cell carcinoma (RCC). Here, the relationship between miR-22 expression pattern and clinicopathological features of patients with EOC were determined by real-time quantitative RT-PCR (qRT-PCR). Furthermore, the role of miR-22 and possible molecular mechanisms in EOC were investigated by several in vitro approaches and in a nude mouse model. Results from qRT-PCR showed that miR-22 was significantly downregulated in RCC samples compared with corresponding non-cancerous tissues, which was significantly associated with tumor stage and lymph node metastasis. Functional study demonstrated that enforced overexpression of miR-22 in renal cancer cells inhibited proliferation, migration and invasion, and induced cell apoptosis in vitro, and suppressed tumor growth in vivo. In addition, SIRT1 was identified as a direct target of miR-22 by a luciferase reporter assay. Overexpression of miR-22 activated p53 and its downstream target p21 and PUMA, and the apoptosis markers cleaved CASP3 and PARP, and inhibited epithelial-mesenchymal transition (EMT). These findings showed that miR-22 functioned as tumor suppressor in RCC and blocked RCC growth and metastasis by directly targeting SIRT1 in RCC, indicating a potential novel therapeutic role in RCC treatment.
引用
收藏
页码:559 / 567
页数:9
相关论文
共 39 条
[1]  
[Anonymous], FEBS J
[2]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29
[3]  
Chen H., 2015, UROL ONCOL-SEMIN ORI, V33, P332
[4]   Sirtuin 1 Is Upregulated in a Subset of Hepatocellular Carcinomas where It Is Essential for Telomere Maintenance and Tumor Cell Growth [J].
Chen, Juan ;
Zhang, Bin ;
Wong, Nathalie ;
Lo, Anthony W. I. ;
To, Ka-Fai ;
Chan, Anthony W. H. ;
Ng, Margaret H. L. ;
Ho, Cecilia Y. S. ;
Cheng, Suk-Hang ;
Lai, Paul B. S. ;
Yu, Jun ;
Ng, Ho-Keung ;
Ling, Ming-Tat ;
Huang, Ai-Long ;
Cai, Xue-Fei ;
Ko, Ben C. B. .
CANCER RESEARCH, 2011, 71 (12) :4138-4149
[5]   Epidemiology and risk factors for kidney cancer [J].
Chow, Wong-Ho ;
Dong, Linda M. ;
Devesa, Susan S. .
NATURE REVIEWS UROLOGY, 2010, 7 (05) :245-257
[6]   Chronic pancreatitis and pancreatic cancer demonstrate active epithelial-mesenchymal transition profile, regulated by miR-217-SIRT1 pathway [J].
Deng, Shichang ;
Zhu, Shuai ;
Wang, Bo ;
Li, Xiang ;
Liu, Yang ;
Qin, Qi ;
Gong, Qiong ;
Niu, Yi ;
Xiang, Cheng ;
Chen, Jingyuan ;
Jin, Yan ;
Deng, Shijiang ;
Yin, Tao ;
Yang, Ming ;
Wu, Heshui ;
Wang, Chunyou ;
Zhao, Gang .
CANCER LETTERS, 2014, 355 (02) :184-191
[7]   Cancer Treatment and Survivorship Statistics, 2014 [J].
DeSantis, Carol E. ;
Lin, Chun Chieh ;
Mariotto, Angela B. ;
Siegel, Rebecca L. ;
Stein, Kevin D. ;
Kramer, Joan L. ;
Alteri, Rick ;
Robbins, Anthony S. ;
Jemal, Ahmedin .
CA-A CANCER JOURNAL FOR CLINICIANS, 2014, 64 (04) :252-271
[8]   Palliative irradiation for focally symptomatic metastatic renal cell carcinoma: Support for dose escalation based on a biological model [J].
DiBiase, SJ ;
Valicenti, RK ;
Schultz, D ;
Xie, Y ;
Gomella, LG ;
Corn, BW .
JOURNAL OF UROLOGY, 1997, 158 (03) :746-749
[9]  
Dong Y. M., 2014, ACTA CHROMATOGR, V1, P1, DOI DOI 10.1007/S13277-014-2631-4
[10]   Regulation of mRNA Translation and Stability by microRNAs [J].
Fabian, Marc Robert ;
Sonenberg, Nahum ;
Filipowicz, Witold .
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 79, 2010, 79 :351-379