Studies on the interaction of heparin with lysozyme by multi-spectroscopic techniques and atomic force microscopy

被引：17
作者：
Tian, Lunfu [1 ,2 ]
Hu, Xiaoli [2 ]
Liu, Zhongfang [2 ]
Liu, Shaopu [2 ]
机构：
[1] China Acad Engn Phys, Inst Mech Mfg Technol, Mianyang 621900, Peoples R China
[2] Southwest Univ, Sch Chem & Chem Engn, Educ Minist, Key Lab Luminescence & Real Time Anal, Chongqing 400715, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 154卷
基金：
中国国家自然科学基金;
关键词：
Heparin; Lysozyme; Interaction; Spectroscopic techniques; Atomic force microscopy; RESONANCE RAYLEIGH-SCATTERING; BOVINE SERUM-ALBUMIN; LIGHT-SCATTERING; QUANTUM DOTS; SILVER NANOPARTICLES; MOLECULAR DOCKING; METHYLENE-BLUE; NANOGRAM LEVEL; BINDING; ASSAY;
D O I：
10.1016/j.saa.2015.09.026
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
The interaction between heparin (Hep) and lysozyme (Lyso) in vitro was studied by fluorescence, UV-vis, circular dichroism (CD), resonance Rayleigh scattering (RRS) spectroscopy and atomic force microscopy (AFM) under normal physiological conditions. UV-vis spectra of Lyso showed the absorbance was significantly increased with the addition of Hep. Fluorescence studies revealed that the emission quenching of Lyso with Hep was initiated by static quenching mechanism. CD spectral studies showed that Hep induced conformational changes in the secondary structure of Lyso. RRS spectra of Lyso showed the intensity of scattering was significantly increased with the addition of Hep and the enhanced RRS intensities were proportional to the concentration of Hep in a certain range. Thus, a new RRS method using Lyso as a probe could be used for the determination of Hep. The detection limit for Hep was 3.9 ng mL(-1). In addition, the shape of the complex was characterized by AFM. The possible reaction mechanism and the reasons for the enhancement of RRS intensity had been discussed through experimental results. (C) 2015 Elsevier BM. All rights reserved.
引用
收藏
页码：27 / 32
页数：6
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