Determination of DNA using sodium 9,10-anthraquinone-2-sulfonate as an in situ photochemical fluorescence probe

被引：31
|
作者：
Li, WY [1 ]
Guo, XQ [1 ]
Xu, JG [1 ]
Zhu, QZ [1 ]
Zhao, YB [1 ]
机构：
[1] XIAMEN UNIV,DEPT CHEM,RES LAB SEDC ANALYT SCI MAT & LIFE CHEM,XIAMEN 361005,PEOPLES R CHINA
来源：
ANALYTICA CHIMICA ACTA | 1997年 / 340卷 / 1-3期
基金：
中国国家自然科学基金;
关键词：
fluorimetry; DNA; photochemical fluorescence probe; sodium 9,10-anthraquinone-2-sulfonate;
D O I：
10.1016/S0003-2670(96)00446-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An in situ photochemical fluorescence probe method for the determination of DNA with sodium 9,10-anthraquinone-2-sulfonate (AQS) as a photochemical fluorescence probe was developed. It was based on the conversion of AQS into an intensively fluorescent product by irradiating with UV radiation. The photochemical reaction is retarded by DNA. The determination can be carried out by measuring the fluorescence intensity at a fixed time. The calibration graph was linear in the range 0-80 ng ml(-1) calf thymus (CT) DNA (r = 0.9991), the limit of detection was 3.2 ng ml(-1) CT DNA (n = 9). The kinetic behaviour of the photochemical reaction and the effects of experimental conditions were investigated and discussed in detail. The results of absorption spectra and competitive binding experiments suggested the interaction between AQS and DNA to be intercalative.
引用
收藏
页码：291 / 296
页数：6
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