Injectable Tissue-Engineered Cartilage Using Commercially Available Fibrin Glue

被引:19
作者
Cakmak, Ozcan [1 ]
Babakurban, Seda T. [9 ]
Akkuzu, Hatice G. [3 ]
Bilgi, Selcuk [2 ]
Ovali, Ercument [4 ,5 ]
Kongur, Merve [4 ,5 ]
Altintas, Hande [6 ]
Yilmaz, Bayram [8 ]
Bilezikci, Banu [10 ]
Celik, Zerrin Y. [11 ]
Yakicier, Mustafa C. [7 ]
Sahin, Feride I. [11 ]
机构
[1] Acibadem Univ, Fac Med, Dept Otolaryngol, Ankara, Turkey
[2] Acibadem Univ, Fac Med, Dept Pathol, Ankara, Turkey
[3] Acibadem Healthcare Grp, Atasehir Surg Med Ctr, Dept Otolaryngol, Ankara, Turkey
[4] Acibadem Healthcare Grp, Labcell Stem Cell Lab, Ankara, Turkey
[5] Acibadem Healthcare Grp, Umbil Cord Blood Bank, Ankara, Turkey
[6] Acibadem Healthcare Grp, Kadikoy Hosp, Dept Otolaryngol, Ankara, Turkey
[7] Acibadem Univ, Fac Med, Dept Med Biol, Ankara, Turkey
[8] Yeditepe Univ, Fac Med, Dept Physiol, Ankara, Turkey
[9] Baskent Univ, Fac Med, Dept Otolaryngol, TR-06490 Ankara, Turkey
[10] Baskent Univ, Fac Med, Dept Pathol, TR-06490 Ankara, Turkey
[11] Baskent Univ, Fac Med Genet, TR-06490 Ankara, Turkey
关键词
Issue engineering; cartilage; fibrin glue; immunocompromised; CHONDROCYTES;
D O I
10.1002/lary.24156
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objectives/HypothesisTo achieve injectable tissue-engineered cartilage using a commercially available fibrin sealant, and to determine the most suitable fibrin glue concentration, cartilage source, and cultured chondrocyte concentration. Study DesignAnimal research. MethodsA total of 28 immuncompetent New Zealand white rabbits were divided into four groups. The cultured chondrocytes from different anatomical sources carried in fibrin glue with and without aprotinin in different concentrations of fibrinogen and thrombin (Tisseell), were injected into forehead and interocular regions of the rabbits. The new tissue formation was harvested at 8 weeks and analyzed through gross and histological analysis. ResultsThe new tissue formations were found in round, elliptical, and flat forms. The mean value of Tisseell and cell suspension was 0.8 cc in all of the rabbits' injection regions, but the mean volume of the samples in which immature cartilage matrix and mature cartilage was 0.1 cc. In the 20 of the 55 injection regions of rabbits (36, 36%), mature and/or immature cartilage formation were observed. We observed inflammatory reactions, abscess formation, and foreign body reactions around the new cartilage tissue of tissue-engineered cartilage. The comparison of results using different cartilage sources, chondrocyte concentrations, or different fibrin glue concentrations did not show any significant difference. ConclusionsWe observed that changing the concentrations of ingredients of commercially available fibrin glue, the source of the cartilage, or the cultured chondrocyte concentration did not have significant effect on neocartilage formation. Level of EvidenceN/A. Laryngoscope, 123:2986-2992, 2013
引用
收藏
页码:2986 / 2992
页数:7
相关论文
共 18 条
  • [1] Cakmak O, 2007, ARCH FACIAL PLAST S, V9, P161
  • [2] Injectable tissue-engineered cartilage using a fibrin sealant
    Chang, Jinsoon
    Rasamny, Jk J.
    Park, Stephen S.
    [J]. ARCHIVES OF FACIAL PLASTIC SURGERY, 2007, 9 (03) : 161 - 166
  • [3] Effects of fibrin glue on nasal septal tissues
    Erkan, Alper Nabi
    Cakmak, Ozcan
    Kocer, Nazim Emrah
    Yilmaz, Ismail
    [J]. LARYNGOSCOPE, 2007, 117 (03) : 491 - 496
  • [4] Institute of Laboratory Animal Resources Commission on Life Sciences National Research Council, 1996, GUIDE CARE USE LAB A, V7th
  • [5] Lu LC, 2001, CLIN ORTHOP RELAT R, pS251
  • [6] PAIGE KT, 1995, PLAST RECONSTR SURG, V96, P1390, DOI 10.1097/00006534-199511000-00024
  • [7] Effects of cell concentration and growth period on articular and ear chondrocyte transplants for tissue engineering
    Panossian, A
    Ashiku, S
    Kirchhoff, CH
    Randolph, MA
    Yaremchuk, MJ
    [J]. PLASTIC AND RECONSTRUCTIVE SURGERY, 2001, 108 (02) : 392 - 402
  • [8] Cartilage generation using alginate-encapsulated autogenous chondrocytes in rabbits
    Park, DJ
    Bong, JP
    Park, SY
    Hong, KS
    [J]. ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 2000, 109 (12) : 1157 - 1161
  • [9] Cultured chondrocytes produce injectable tissue-engineered cartilage in hydrogel polymer
    Passaretti, D
    Silverman, RP
    Huang, W
    Kirchhoff, CH
    Ashiku, S
    Randolph, MA
    Yaremchuk, MJ
    [J]. TISSUE ENGINEERING, 2001, 7 (06): : 805 - 815
  • [10] Review of injectable cartilage engineering using fibrin gel in mice and swine models
    Peretti, Giuseppe M.
    Xu, Jian-Wei
    Bonassar, Lawrence J.
    Kirchhoff, Carl Hendrick
    Yaremchuk, Michael J.
    Randolph, Mark A.
    [J]. TISSUE ENGINEERING, 2006, 12 (05): : 1151 - 1168