Octreotide inhibits growth of colonic cancer SW480 cells by modulating the Wnt/β-catenin pathway

被引：14

作者：

Chen, Jing-song ^{[1
]}

Liang, Qing-mo ^{[1
]}

Li, Hua-shu ^{[2
]}

Yang, Jie ^{[1
]}

Wang, Song ^{[1
]}

Long, Jian-wu ^{[1
]}

机构：

[1] Univ S China, Affiliated Nanhua Hosp, Dept Oncol, Hengyang 421002, Hunan, Peoples R China

[2] 169th Cent Hosp Peoples Liberat Army, Dept Obstet & Gynecol, Hengyang, Hunan, Peoples R China

来源：

PHARMAZIE | 2009年 / 64卷 / 02期

关键词：

PITUITARY-TUMOR CELLS; TCF SIGNALING PATHWAY; BETA-CATENIN; POSITIVE REGULATOR; COLORECTAL-CANCER; CARCINOMA CELLS; GASTRIC-CANCER; CYCLIN D1; PROTEIN; PHOSPHORYLATION;

D O I：

10.1691/ph.2009.8678

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Somatostatin can suppress the growth of various tumor cells including colonic cancer. Activated Wnt/beta-catenin signaling pathway plays a critical role in tumorgenesis and development of colorectal cancer. However, the effect of somatostatin on Wnt/beta-catenin signaling pathway remains unknown. Thus, we investigated the effect of octreotide on Wnt/beta-catenin signaling pathway in human colonic cancer cell SW480. The results of 3-(4,5-imethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and flow cytometric assays showed that octreotide inhibited growth, induced apoptosis and arrested the G, cell cycle of SW480 cells in a dose-dependent manner. We demonstrated that octreotide significally up-regulated and down-regulated 13 genes and 17 genes in Wnt/beta-catenin signaling using microarray, respectively. Furthermore, as evidenced by western blot, beta-catenin protein level decreased, whereas phosphorylated P-catenin protein level increased under octreotide. The present study reveals that octreotide can inhibit human colonic cancer cell growth through inhibition of Wnt/beta-catenin signaling pathway.

引用

页码：126 / 131

页数：6

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