A complex of the soluble interleukin-6 receptor and interleukin-6 is internalized via the signal transducer gp130

被引：26
作者：
Graeve, L
Korolenko, TA
Hemmann, U
Weiergraber, O
Dittrich, E
Heinrich, PC
机构：
[1] Institut für Biochemie, Rheinisch-Westfalische TH Aachen, 52057 Aachen
来源：
FEBS LETTERS | 1996年 / 399卷 / 1-2期
关键词：
interleukin-6; soluble interleukin-6 receptor; gp130; endocytosis; lysosome;
D O I：
10.1016/S0014-5793(96)01305-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In human body fluids a soluble form of the interleukin-6 receptor (sIL-6R) has been found which together with interleukin-6 (IL-6) acts agonistically on cells expressing the signal transducer gp130. The means by which the sIL-6R is removed from the circulation is unknown. Here, we show that a complex of I-125-labelled recombinant sIL-6R and IL-6 is internalized by MDCK cells stably transfected with gp130 and by human hepatoma cells HepG2 that endogenously express the IL-6R and gp130. We further show that most of the internalized sIL-6R is degraded within lysosomes. Our studies suggest that cells expressing gp130 are capable of endocytosing an IL-6/s-IL-6R complex, thereby removing both from the circulation.
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收藏
页码：131 / 134
页数：4
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