Magnetic cross-linked enzyme aggregates of Km12 lipase: A stable nanobiocatalyst for biodiesel synthesis from waste cooking oil

被引：62
作者：
Badoei-dalfard, Arastoo [1 ,2 ]
Malekabadi, Saeid [1 ]
Karami, Zahra [1 ]
Sargazi, Ghasem [3 ]
机构：
[1] Shahid Bahonar Univ Kerman, Fac Sci, Dept Biol, Kerman, Iran
[2] Shahid Bahonar Univ Kerman, RTIPP, Kerman, Iran
[3] Grad Univ Adv Technol, Dept Nanotechnol, Kerman, Iran
来源：
RENEWABLE ENERGY | 2019年 / 141卷
关键词：
mCLEAs; Lipase; Biodiesel; Waste cooking oil; Renewable fuel; SOLVENT-TOLERANT LIPASE; IMMOBILIZED LIPASE; SILICA-GEL; PYROLYSIS; TRANSESTERIFICATION; NANOPARTICLES; PURIFICATION; CONVERSION; ENERGY; MICROSPHERES;
D O I：
10.1016/j.renene.2019.04.061
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Enzymatic production of biodiesel from waste cooking oils (WCOs) is expected as an efficient procedure for resolving the problems of energy demand and environment pollutions. But, high cost of lipases has been found as a main obstacle to commercialize the enzymatic transesterification. In the present study, cross-linked enzyme aggregates of Km12 lipases have been coupled with amino coated magnetite nanoparticles. SEM analysis showed that mCLEAs-lipase (mCLEAs-lip) nanocomposites have spherical structures. The mCLEAs-lip displayed a shift in optimal pH towards the alkaline, whereas optimal temperature was also shift towards low temperature. mCLEAs-lip nanocomposite reserves its total activity up to 6 cycles of enzyme re-using. Furthermore, it reserves 60% of its initial activity more than free enzyme after 24 days of incubation at 4 degrees C. Biodiesel production from waste cooking oils by immobilized enzyme increased about 20% more than free enzyme. Therefore, the present study displays great practical latent to produce renewable fuel such as biodiesel. (C) 2019 Elsevier Ltd. All rights reserved.
引用
收藏
页码：874 / 882
页数：9
相关论文
共 64 条
[1]   Biodiesel production from waste cooking oil using onsite produced purified lipase from Pseudomonas aeruginosa FW_SH-1: Central composite design approach [J].
Ali, Chaudhry Haider ;
Qureshi, Abdul Sattar ;
Mbadinga, Serge Maurice ;
Liu, Jin-Feng ;
Yang, Shi-Zhong ;
Mu, Bo-Zhong .
RENEWABLE ENERGY, 2017, 109 :93-100
[2]   A comprehensive review on biodiesel as an alternative energy resource and its characteristics [J].
Atabani, A. E. ;
Silitonga, A. S. ;
Badruddin, Irfan Anjum ;
Mahlia, T. M. I. ;
Masjuki, H. H. ;
Mekhilef, S. .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2012, 16 (04) :2070-2093
[3]   Preparation of cross-linked tyrosinase aggregates [J].
Aytar, Burcu Selin ;
Bakir, Ufuk .
PROCESS BIOCHEMISTRY, 2008, 43 (02) :125-131
[4]  
Azadian Fatemeh, 2017, Journal of Genetic Engineering and Biotechnology, V15, P187, DOI 10.1016/j.jgeb.2016.12.005
[5]  
Azadian F, 2016, MOL BIOL RES COMMUN, V5, P143
[6]   Enzymatic production of biodiesel using lipases immobilized on silica nanoparticles as highly reusable biocatalysts: effect of water, t-butanol and blue silica gel contents [J].
Babaki, Mohadese ;
Yousefi, Maryam ;
Habibi, Zohreh ;
Mohammadi, Mehdi ;
Yousefi, Parisa ;
Mohammadi, Javad ;
Brask, Jesper .
RENEWABLE ENERGY, 2016, 91 :196-206
[7]   Construction of CLEAs-lipase on magnetic graphene oxide nanocomposite: An efficient nanobiocatalyst for biodiesel production [J].
Badoei-dalfard, Arastoo ;
Karami, Zahra ;
Malekabadi, Saied .
BIORESOURCE TECHNOLOGY, 2019, 278 :473-476
[8]   Purification and Characterization of a Thermo- and Organic Solvent-Tolerant Alkaline Protease from Bacillus sp. JER02 [J].
Badoei-Dalfard, Arastoo ;
Karami, Zahra ;
Ravan, Hadi .
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2015, 45 (02) :128-143
[9]   Prospects of biodiesel production from vegetables oils in India [J].
Barnwal, BK ;
Sharma, MP .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2005, 9 (04) :363-378
[10]   High performance heterogeneous catalyst for biodiesel production from vegetal and waste oil at low temperature [J].
Borges, M. E. ;
Diaz, L. ;
Alvarez-Galvan, M. C. ;
Brito, A. .
APPLIED CATALYSIS B-ENVIRONMENTAL, 2011, 102 (1-2) :310-315
1234567