Differential expression of cGMP-inhibited cyclic nucleotide phosphodiesterases in human hepatoma cell lines

被引：13

作者：

Murata, T ^{[1
]}

Taira, M ^{[1
]}

Manganiello, VC ^{[1
]}

机构：

[1] NHLBI,PULM CRIT CARE MED BRANCH,NIH,BETHESDA,MD 20892

来源：

FEBS LETTERS | 1996年 / 390卷 / 01期

关键词：

Human hepatoma cell line; PDE3 or cGMP-inhibited cyclic nucleotide phosphodiesterase; PDE4 or cAMP-specific phosphodiesterase;

D O I：

10.1016/0014-5793(96)00410-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PDE3 or cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI PDE) activity was detected in homogenates of HepG2, Hep3B and HuH7, but not SK-Hep-1, human hepatoma cells, In HepG2 and Hep3B cells PDE3 activity was found predominantly in particulate fractions; in HuH7, in both particulate and supernatant fractions. cDNAs encoding two human PDE3s (an 'adipocyte' type, HcGIP1, and a 'cardiovascular' type, HcGIP2) have been cloned, HcGIP1 cDNA hybridized strongly with poly(A)(+) RNA species from HepG2 and Hep3B, Both HcGIP1 and HcGIP2 mRNAs were expressed in Hep3B and HuH7 cells, The nucleotide sequence of an similar to 300-bp cDNA fragment, isolated after RT-PCR cloning from HepG2 RNA, was identical to a sequence within the conserved domain of HcGIP1 cDNA, consistent with the presence of HcGIP1 mRNA in HepG2 cells.

引用

页码：29 / 33

页数：5

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