Acetylcholine-induced vasodilation and reactive hyperemia are not affected by acute cyclo-oxygenase inhibition in human skin

被引:29
作者
Dalle-Ave, A [1 ]
Kubli, S [1 ]
Golay, S [1 ]
Delachaux, A [1 ]
Liaudet, L [1 ]
Waeber, B [1 ]
Feihl, F [1 ]
机构
[1] Univ Hosp, Div Clin Pathophysiol & Med Teaching, CH-1011 Lausanne, Switzerland
关键词
acetylcholine; aspirin; endothelial function; indomethacin; iontophoresis; prostanoids; skin blood flow; hyperemic response;
D O I
10.1080/10739680490449268
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To examine whether prostaglandins are involved in endothelium-dependent vasodilatory responses of the skin microcirculation. Methods: Twenty-three young male volunteers were studied on 2 different days 1-3 weeks apart. On each experimental day the forearm skin blood flow response to iontophoretically applied acetylcholine (Ach, an endothelium-dependent vasodilator) was determined with laser Doppler imaging following the intravenous administration of either the cyclo-oxygenase inhibitor lysine acetylsalicylate (L-AS), 900 mg, or the oral intake of indomethacin, 75 mg. Acetylcholine was iontophoresed both in presence and in absence of surface anesthesia. In some subjects, the effects of L-AS on skin reactive hyperemia were also assessed. Results: Acute cyclo-oxygenase inhibition with either drug influenced neither the skin blood flow response to 4 different doses of Ach (0.28, 1.4, 7, and 14 mC/cm(2)) nor reactive hyperemia. The peak vasodilatory response to Ach was significantly increased by skin anesthesia, regardless of whether the subjects received the cyclo-oxygenase inhibitor or not. For example, the mean response (+/-SD) to the largest dose of Ach (tested in 6 subjects, expressed in perfusion units) were as follows: in absence of anesthesia: L-AS 339 +/- 105, placebo 344 +/- 68; with anesthesia: L-AS 453 +/- 76, placebo 452 +/- 65 (p < .01 for effect of anesthesia). Conclusions: These data give no support for a contribution of prostaglandins to acetylcholine-induced vasodilation or to reactive hyperemia in the skin microcirculation. In this vascular bed, local anesthesia seems to amplify acetylcholine-induced vasodilation via a prostaglandin-independent mechanism.
引用
收藏
页码:327 / 336
页数:10
相关论文
共 34 条
[1]   The effect of aspirin and various iontophoresis solution vehicles on skin microvascular reactivity [J].
Abou-Elenin, K ;
Xydakis, A ;
Hamdy, O ;
Economides, PA ;
Horton, ES ;
Veves, A .
MICROVASCULAR RESEARCH, 2002, 63 (01) :91-95
[2]  
Andreassen AK, 1998, CLIN TRANSPLANT, V12, P324
[3]   Estrogen improves endothelial function - Discussion [J].
Donaldson, MC ;
Arora, S ;
Kaufman, JL ;
Cronenwett, JL ;
McDaniel, MD ;
Whittmore, A ;
Babson, WW .
JOURNAL OF VASCULAR SURGERY, 1998, 27 (06) :1147-1147
[4]   Vascular and neural mechanisms of ACh-mediated vasodilation in the forearm cutaneous microcirculation [J].
Berghoff, M ;
Kathpal, M ;
Kilo, S ;
Hilz, MJ ;
Freeman, R .
JOURNAL OF APPLIED PHYSIOLOGY, 2002, 92 (02) :780-788
[5]   Microvascular and macrovascular reactivity is reduced in subjects at risk for type 2 diabetes [J].
Caballero, AE ;
Arora, S ;
Saouaf, R ;
Lim, SC ;
Smakowski, P ;
Park, JY ;
King, GL ;
LoGerfo, FW ;
Horton, ES ;
Veves, A .
DIABETES, 1999, 48 (09) :1856-1862
[6]  
Celermajer DS, 1998, J CARDIOVASC PHARM, V32, pS29
[7]   ANTIDROMIC VASODILATATION AND NEUROGENIC INFLAMMATION [J].
CHAHL, LA .
PHARMACOLOGY & THERAPEUTICS, 1988, 37 (02) :275-300
[8]   Contribution of nitric oxide and prostaglandins to reactive hyperemia in the human forearm [J].
Engelke, KA ;
Halliwill, JR ;
Proctor, DN ;
Dietz, NM ;
Joyner, MJ .
JOURNAL OF APPLIED PHYSIOLOGY, 1996, 81 (04) :1807-1814
[9]  
FLOWER RJ, 1985, PHARMACOL BASIS THER, P674
[10]   RESOLUTION OF EXPERIMENTAL MICROVASCULAR SPASM AND IMPROVEMENT IN ANASTOMOTIC PATENCY BY DIRECT TOPICAL AGENT APPLICATION [J].
GETER, RK ;
WINTERS, RRW ;
PUCKETT, CL .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1986, 77 (01) :105-115