Fluorescent BODIPY-labelled GM1 gangliosides designed for exploring lipid membrane properties and specific membrane-target interactions

被引：19

作者：

Mikhalyov, Ilya ^{[2
]}

Gretskaya, Natalia ^{[2
]}

Johansson, Lennart B. -A. ^{[1
]}

机构：

[1] Umea Univ, Dept Chem, S-90187 Umea, Sweden

[2] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2009年 / 159卷 / 01期

基金：

瑞典研究理事会;

关键词：

BODIPY-labelled gangliosides; G(M1) ganglioside; Forster energy transfer; DIPYRROMETHENEBORON DIFLUORIDE BODIPY; TOXIN-B-SUBUNIT; SPECTROSCOPY; AGGREGATION; PEPTIDE; PROBES; SEPARATION; BILAYERS; SURFACE; PHASES;

D O I：

10.1016/j.chemphyslip.2009.01.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New fluorophore-labelled Gm, gangliosides have been synthesised and spectroscopically characterised. Spectroscopically different BODIPY groups were covalently linked, specifically to either the polar or the hydrophobic part of the ganglioside molecule. The absorption and fluorescence spectroscopic properties are reported for 564/571-BODIPY- and 581/591-BODIPY-labelled G(M1). Each of the different BODIPY groups is highly fluorescent and depolarisation experiments provide molecular information about the spatial distribution in lipid bilayers, as well as order and dynamics. From experiments performed on two spectroscopically different BODIPY:s. specific interactions can be revealed by monitoring the rate/efficiency of donor-acceptor electronic energy transfer. Systems of particular interest for applying these probes are e.g. mixtures of lipids, and peptides/proteins interacting with lipid membranes. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：38 / 44

页数：7

共 5 条

[1] Designed Fluorescent Probes Reveal Interactions between Amyloid-β(1-40) Peptides and GM1 Gangliosides in Micelles and Lipid Vesicles
Mikhalyov, I.
Olofsson, A.
Grobner, G.
Johansson, L. B. -A.
BIOPHYSICAL JOURNAL, 2010, 99 (05) : 1510 - 1519
[2] Influence of Ganglioside GM1 Concentration on Lipid Clustering and Membrane Properties and Curvature
Patel, Dhilon S.
Park, Soohyung
Wu, Emilia L.
Yeom, Min Sun
Widmalm, Goran
Klauda, Jeffery B.
Im, Wonpil
BIOPHYSICAL JOURNAL, 2016, 111 (09) : 1987 - 1999
[3] Lipid domains in the membrane: Thermotropic properties of sphingomyelin vesicles containing GM1 ganglioside and cholesterol
Ferraretto, A
Pitto, M
Palestini, P
Masserini, M
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[4] Segregation of gangliosides GM1 and GD3 on cell membranes, isolated membrane rafts, and defined supported lipid monolayers
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Patel, HV
Vyas, AA
Schnaar, RL
BIOLOGICAL CHEMISTRY, 2001, 382 (02) : 241 - 250
[5] INCREASED INSULIN RECEPTOR AND DECREASED GM1 EXPRESSION IN MEMBRANE LIPID RAFT OF PATIENTS WITH NASH: A POTENTIAL BIOMARKER AND THERAPEUTIC TARGET
Lalazar, Gadi
Ben-Ya'acov, Ami
Admon, Ester
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HEPATOLOGY, 2008, 48 (04) : 846A - 846A

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