RETRACTED: Chitosan tripolyphosphate (CS/TPP) nanoparticles: Preparation, characterization and application for gene delivery in shrimp(Retracted article. See vol. 168, pg. 91, 2017)

被引：89
作者：
Vimal, S. [1 ]
Majeed, S. Abdul [1 ]
Taju, G. [1 ]
Nambi, K. S. N. [1 ]
Raj, N. Sundar [1 ]
Madan, N. [1 ]
Farook, M. A. [1 ]
Rajkumar, T. [1 ]
Gopinath, D. [1 ]
Hameed, A. S. Sahul [1 ]
机构：
[1] C Abdul Hakeem Coll, OIE Reference Lab WTD, Aquaculture Biotechnol Div, PG & Res Dept Zool, Melvisharam 632509, Tamil Nadu, India
来源：
ACTA TROPICA | 2013年 / 128卷 / 03期
关键词：
CS/TPP nanoparticles; pVP28; DNA; In vivo gene expression; Real-time PCR; Immuno-dot blot; PROTEIN DELIVERY; MOLECULAR-WEIGHT; ORAL DELIVERY; CONTROLLED-RELEASE; DNA NANOPARTICLES; SYSTEM; CARRIERS; VACCINE; MICROSPHERES; ABSORPTION;
D O I：
10.1016/j.actatropica.2013.07.013
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The present study examines the use of CS/TPP nanoparticles for gene delivery in different tissues of shrimp through oral route. The viral gene of WSSV was used to construct DNA vaccines using pcDNA 3.1, a eukaryotic expression vector and the constructs were named as pVP28. The CS/TPP nanoparticles were synthesized by ionic gelation process and these particles were characterized. The structure and morphology of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT assay using fish cell line. The expression of gene was confirmed by Immuno-dot blot, ELISA and RT-PCR analyses. The results indicate that DNA can be easily delivered into shrimp by feeding with CS/TPP nanoparticles. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：486 / 493
页数：8
相关论文
共 50 条
[41]   RETRACTED: Fully automatic synthesis of [18F]FES for reporter gene hERL expression imaging (Retracted article. See vol. 33, pg. 562, 2012) [J].
Liang, Sheng ;
Lan, Xiaoli ;
Zhang, Yongxue ;
Xu, Xuemin ;
Li, Biao .
NUCLEAR MEDICINE COMMUNICATIONS, 2012, 33 (01) :29-33
[42]   RETRACTED: Fresh water production from/by atmospheric air for arid regions, using solar energy: Review (Retracted article. See vol. 22, pg. 735, 2013) [J].
El-Ghonemy, A. M. K. .
RENEWABLE & SUSTAINABLE ENERGY REVIEWS, 2012, 16 (08) :6384-6422
[43]   RETRACTED: Novel drug delivery system based on doxorubicin-encapsulated magnetic nanoparticles modified with PLGA-PEG1000 copolymer (Retracted article. See vol. 50, pg. 109, 2022) [J].
Ebrahimi, Eommolbanin ;
Akbarzadeh, Abolfazl ;
Abbasi, Elham ;
Khandaghi, Amir Ahmad ;
Abasalizadeh, Farhad ;
Davaran, Soodabeh .
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2016, 44 (01) :290-297
[44]   RETRACTED: The cortical representation of the speech envelope is earlier for audiovisual speech than audio speech (Retracted article. See vol. 112, pg. 2667, 2014) [J].
Crosse, Michael J. ;
Lalor, Edmund C. .
JOURNAL OF NEUROPHYSIOLOGY, 2014, 111 (07) :1400-1408
[45]   RETRACTED: Assessing and validating tourism business model in hospitality industry: role of blockchain platform (Retracted article. See vol. 31, pg. 58208, 2024) [J].
Cheng, Ruifen .
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2023, 30 (23) :63704-63715
[46]   RETRACTED: Ondansetron loaded pectin based microspheres for nasal administration: In vitro and in vivo studies (Retracted article. See vol. 342, pg. 1016, 2019) [J].
Mahajan, Hitendra S. ;
Tatiya, Bhushankumar V. ;
Nerkar, Pankaj P. .
POWDER TECHNOLOGY, 2012, 221 :168-176
[47]   RETRACTED: Neurotrophin 3 Transduction Augments Remyelinating and Immunomodulatory Capacity of Neural Stem Cells (Retracted article. See vol. 31, pg. 2552, 2023) [J].
Yang, Jingxian ;
Yan, Yaping ;
Xia, Yang ;
Kang, Tingguo ;
Li, Xing ;
Ciric, Bogoljub ;
Xu, Hui ;
Rostami, Abdolmohamad ;
Zhang, Guang-Xian .
MOLECULAR THERAPY, 2014, 22 (02) :440-450
[48]   RETRACTED: Wind turbine power coefficient estimation by soft computing methodologies: Comparative study (Retracted article. See vol. 159, pg. 414, 2018) [J].
Shamshirband, Shahaboddin ;
Petkovic, Dalibor ;
Saboohi, Hadi ;
Anuar, Nor Badrul ;
Inayat, Irum ;
Akib, Shatirah ;
Cojbasic, Zarko ;
Nikolic, Vlastimir ;
Kiah, Miss Laiha Mat ;
Gani, Abdullah .
ENERGY CONVERSION AND MANAGEMENT, 2014, 81 :520-526
[49]   RETRACTED: Information distortion in a supply chain and its mitigation using soft computing approach (Retracted article. See vol. 40, pg. 502, 2012) [J].
Balan, S. ;
Vrat, Prem ;
Kumar, Pradeep .
OMEGA-INTERNATIONAL JOURNAL OF MANAGEMENT SCIENCE, 2009, 37 (02) :282-299
[50]   RETRACTED: Absolute quantification of free tumor cells in the peripheral blood of gastric cancer patients (Retracted article. See vol. 14, pg. 789, 2015) [J].
Bayat, N. ;
Mokhtari, M. M. ;
Rezaei-Tavirani, M. ;
Baradaran-rafii, A. ;
Zadeh, S. Rahman ;
Heidari-Keshel, S. ;
Ghasemvand, F. .
GENETICS AND MOLECULAR RESEARCH, 2014, 13 (02) :4425-4432
12345