RETRACTED: Chitosan tripolyphosphate (CS/TPP) nanoparticles: Preparation, characterization and application for gene delivery in shrimp(Retracted article. See vol. 168, pg. 91, 2017)

被引：89
作者：
Vimal, S. [1 ]
Majeed, S. Abdul [1 ]
Taju, G. [1 ]
Nambi, K. S. N. [1 ]
Raj, N. Sundar [1 ]
Madan, N. [1 ]
Farook, M. A. [1 ]
Rajkumar, T. [1 ]
Gopinath, D. [1 ]
Hameed, A. S. Sahul [1 ]
机构：
[1] C Abdul Hakeem Coll, OIE Reference Lab WTD, Aquaculture Biotechnol Div, PG & Res Dept Zool, Melvisharam 632509, Tamil Nadu, India
来源：
ACTA TROPICA | 2013年 / 128卷 / 03期
关键词：
CS/TPP nanoparticles; pVP28; DNA; In vivo gene expression; Real-time PCR; Immuno-dot blot; PROTEIN DELIVERY; MOLECULAR-WEIGHT; ORAL DELIVERY; CONTROLLED-RELEASE; DNA NANOPARTICLES; SYSTEM; CARRIERS; VACCINE; MICROSPHERES; ABSORPTION;
D O I：
10.1016/j.actatropica.2013.07.013
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The present study examines the use of CS/TPP nanoparticles for gene delivery in different tissues of shrimp through oral route. The viral gene of WSSV was used to construct DNA vaccines using pcDNA 3.1, a eukaryotic expression vector and the constructs were named as pVP28. The CS/TPP nanoparticles were synthesized by ionic gelation process and these particles were characterized. The structure and morphology of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT assay using fish cell line. The expression of gene was confirmed by Immuno-dot blot, ELISA and RT-PCR analyses. The results indicate that DNA can be easily delivered into shrimp by feeding with CS/TPP nanoparticles. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：486 / 493
页数：8
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