Separation and simultaneous determination of water-soluble and fat-soluble vitamins by electrokinetic capillary chromatography

被引：32
作者：
Delgado-Zamarreño, MM [1 ]
González-Maza, I [1 ]
Sánchez-Pérez, A [1 ]
Carabias-Martinez, R [1 ]
机构：
[1] Univ Salamanca, Fac Ciencias Quim, Dept Quim Analit Nutr & Bromatol, E-37008 Salamanca, Spain
来源：
JOURNAL OF CHROMATOGRAPHY A | 2002年 / 953卷 / 1-2期
关键词：
electrokinetic capillary chromatography; buffer composition; vitamins; surfactants; bis(2-ethylhexyl) sodium sulfosuccinate;
D O I：
10.1016/S0021-9673(02)00130-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A comparative study of different surfactants, such as sodium dodecyl sulfate and bis(2-ethylhexyl) sodium sulfosuccinate (AOT), as pseudostationary phases was conducted in order to find the best conditions for the separation of the water- and fat-soluble vitamins by electrokinetic capillary chromatography. Separation was accomplished with AOT in a water-acetonitrile solution in the presence of boric acid-sodium borate buffer. A study of different variables was performed in order to obtain the best resolution and quantification of the vitamins: the variables explored were the pH and concentration of the buffer used, the percentage of acetonitrile, the concentration of surfactant and the applied voltage. Calibration curves and precision data were obtained for each analyte, Finally, a method to analyze these substances in pharmaceutical preparations is proposed, (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：257 / 262
页数：6
相关论文
共 17 条
  • [1] Application of microemulsion electrokinetic chromatography to the analysis of a wide range of pharmaceuticals and excipients
    Altria, KD
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 844 (1-2) : 371 - 386
  • [2] USE OF CAPILLARY ELECTROPHORESIS FOR THE DETERMINATION OF VITAMINS OF THE B-GROUP IN PHARMACEUTICAL PREPARATIONS
    BOONKERD, S
    DETAEVERNIER, MR
    MICHOTTE, Y
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 670 (1-2) : 209 - 214
  • [3] MICROEMULSION ELECTROKINETIC CHROMATOGRAPHY WITH DIFFERENT ORGANIC MODIFIERS - SEPARATION OF WATER-SOLUBLE AND LIPID-SOLUBLE VITAMINS
    BOSO, RL
    BELLINI, MS
    MIKSIK, I
    DEYL, Z
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 709 (01) : 11 - 19
  • [4] Micellar electrokinetic chromatography with bis(2-ethylhexyl)sodium sulfosuccinate vesicles -: Determination of synthetic food antioxidants
    Delgado-Zamarreño, MM
    Sánchez-Pérez, A
    Maza, IG
    Hernández-Méndez, J
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 871 (1-2) : 403 - 414
  • [5] Duong LTH, 2000, J PHARMACEUT BIOMED, V22, P879, DOI 10.1016/S0731-7085(00)00298-3
  • [6] Determination of six water-soluble vitamins in a pharmaceutical formulation by capillary electrophoresis
    Fotsing, L
    Fillet, M
    Bechet, I
    Hubert, P
    Crommen, J
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 15 (08) : 1113 - 1123
  • [7] Elimination of adsorption effects in the analysis of water-soluble vitamins in pharmaceutical formulations by capillary electrophoresis
    Fotsing, L
    Fillet, M
    Chiap, P
    Hubert, P
    Crommen, J
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 853 (1-2) : 391 - 401
  • [8] FUJIWARA S, 1988, J CHROMATOGR, V447, P133, DOI 10.1016/S0021-9673(01)91462-1
  • [9] Micellar electrokinetic capillary chromatography analysis of water-soluble vitamins
    Gomis, DB
    González, LL
    Alvarez, DG
    [J]. ANALYTICA CHIMICA ACTA, 1999, 396 (01) : 55 - 60
  • [10] Separation of retinoids by micellar electrokinetic capillary chromatography
    Hsieh, YZ
    Kuo, KL
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 761 (1-2) : 307 - 313
12