Electrochemical-Surface Enhanced Raman Spectroscopic (EC-SERS) Study of 6-Thiouric Acid: A Metabolite of the Chemotherapy Drug Azathioprine

被引：61

作者：

Greene, B. H. C. ^{[1
]}

Alhatab, D. S. ^{[1
]}

Pye, C. C. ^{[1
]}

Brosseau, C. L. ^{[1
]}

机构：

[1] St Marys Univ, Dept Chem, Halifax, NS B3H 3C3, Canada

来源：

JOURNAL OF PHYSICAL CHEMISTRY C | 2017年 / 121卷 / 14期

基金：

加拿大自然科学与工程研究理事会;

关键词：

URIC-ACID; AU NANOPARTICLES; XANTHINE-OXIDASE; 6-MERCAPTOPURINE; DESORPTION; MONOLAYERS; AU(111); SAMPLES; SYSTEM; CELLS;

D O I：

10.1021/acs.jpcc.7b01179

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

6-Thiouric acid (6-TUA) has the potential to be an important biomarker for the action of 6-mercaptopurine (6-MP), an immunosuppressive drug used in patients suffering from acute lymphoblastic leukemia (ALL). 6-TUA, a nonactive metabolite of 6-MP, is excreted in the urine, and routine monitoring of this metabolite can be useful in assessing the efficacy of 6-MP for immune system suppression in patients who have undergone stem cell replacement. In this work, electrochemical surface-enhanced Raman spectroscopy (EC-SERS) is used for the first time to study the adsorption and electrochemical behavior of 6-TUA at a nanostructured silver electrode surface, in both 0.1 M NaF and synthetic urine as supporting electrolytes. In addition, ab initio calculations were completed in an effort to understand the adsorption behavior. It was found that EC-SERS provided excellent signal for 6-TUA down to mu M concentrations in synthetic urine and highlights the future potential of EC-SERS for rapid detection of important urine biomarkers at the patient point-of-care.

引用

页码：8084 / 8090

页数：7

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