Putative biomarkers of malignant transformation of sinonasal inverted papilloma into squamous cell carcinoma

被引：16

作者：

Yang, Zheng ^{[1
,2
]}

Zhang, Yang ^{[1
,2
]}

Wang, Xiangdong ^{[1
,2
]}

Huang, Junwei ^{[1
,2
]}

Guo, Wei ^{[1
,2
]}

Wei, Peng ^{[3
]}

Li, Guojun ^{[4
,5
]}

Wang, Ziqiao ^{[3
]}

Huang, Zhigang ^{[1
,2
]}

Zhang, Luo ^{[1
,2
]}

机构：

[1] Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dong Jiao Min Xiang St, Beijing 100730, Peoples R China

[2] Minist Educ, Key Lab Otolaryngol Head & Neck Surg, Beijing, Peoples R China

[3] Univ Texas MD Anderson Canc Ctr, Dept Biostat, Houston, TX 77030 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Head & Neck Surg, Houston, TX 77030 USA

[5] Univ Texas MD Anderson Canc Ctr, Dept Epidemiol, Houston, TX 77030 USA

来源：

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH | 2019年 / 47卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Sinonasal inverted papilloma; squamous cell carcinoma; methylation; C-phosphate-G islands; epigenetics; INTRAGENIC DNA METHYLATION; GENE-EXPRESSION; LUNG-CANCER; INVASION; PROLIFERATION; MICRORNA-661; PROGRESSION; PLECTIN; TARGET; GLIOMA;

D O I：

10.1177/0300060519838385

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective To compare genome-wide DNA methylation between samples of sinonasal inverted papilloma (SNIP) and squamous cell carcinoma (SCC) samples in order to identify aberrantly methylated genes that might be involved in malignant transformation. Methods Tissue samples were collected from patients. DNA methylation in C-phosphate-G islands and gene promoters was analysed using a DNA methylation microarray kit. The levels of mRNA or protein from aberrantly methylated genes were measured using real-time polymerase chain reaction or Western blot analysis. Results A total of 27 tissue samples were included in this study; 15 SNIP samples and 12 SCCs arising in SNIPs. A total of 11 201 nominally differentially methylated sites were observed between SNIP and SCC arising in SNIPs. Six sites were significantly different at P < 0.01 and contained three genes (MIR661, PLEC and OPA3). These three genes were hypermethylated. In addition, the levels of mature miR-661 mRNA and PLEC protein were significantly upregulated in SCC tissues compared with SNIP samples. The levels of OPA3 protein were downregulated in SCC tissues compared with SNIP samples. Conclusions This study demonstrated hypermethylation and abnormal expression of the MIR661, PLEC and OPA3 genes, suggesting a role for their involvement in the malignant transformation of SNIP.

引用

页码：2371 / 2380

页数：10

共 37 条

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