Improved Testing of Recent HIV-1 Infections with the BioRad Avidity Assay Compared to the Limiting Antigen Avidity Assay and BED Capture Enzyme Immunoassay: Evaluation Using Reference Sample Panels from the German Seroconverter Cohort

被引:32
作者
Hauser, Andrea [1 ]
Santos-Hoevener, Claudia [2 ]
Meixenberger, Karolin [1 ]
Zimmermann, Ruth [2 ]
Somogyi, Sybille [1 ]
Fiedler, Stefan [1 ]
Hofmann, Alexandra [2 ]
Bartmeyer, Barbara [2 ]
Jansen, Klaus [2 ]
Hamouda, Osamah [2 ]
Bannert, Norbert [1 ]
Kuecherer, Claudia [1 ]
机构
[1] Robert Koch Inst, Div HIV & Other Retroviruses, Berlin, Germany
[2] Robert Koch Inst, Div HIV AIDS STI & Blood Borne Infect, Berlin, Germany
来源
PLOS ONE | 2014年 / 9卷 / 06期
关键词
SUBTYPE-E INFECTION; TYPE-1; INCIDENCE; UNITED-STATES; IDENTIFICATION; ANTIBODIES; INDEX; INDIVIDUALS; SPECIFICITY; ACCURACY; ADULTS;
D O I
10.1371/journal.pone.0098038
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: The variety and limitations of current laboratory methods for estimating HIV-incidence has driven attempts to improve and standardize the performance of serological 'Tests for Recent HIV-Infections' (TRI). Primary and follow-up HIV-1 positive plasma samples from individuals with well-defined dates of infection collected as part of the German Seroconverter Cohort provided specimens highly suitable for use in comparing the performance of three TRIs: the AWARE (TM) BED (TM) EIA HIV-1 Incidence test (BED-CEIA), Genetic systems HIV-1/HIV-2 Plus O EIA antibody avidity-based assay (BioRad Avidity) and Sedia (TM) HIV-1 LAg Avidity EIA (LAg Avidity). Methods: The evaluation panel included 180 specimens: 44 from antiretroviral (ARV)-naive individuals with recently acquired HIV-infection (<= 130 days; 25 B and 19 non-B subtypes) and 136 from long-term (. 12 months) infected individuals [ 101 ARV-naive subtype B, 16 non-B subtypes, 14 ARV-treated individuals, 5 slow progressors (SLP)]. Results: For long-term infected, ARV-naive individuals the false recent rates (FRR) of both the BioRad and LAg Avidity assays were 2% (2/101 for subtype B) and 6% (1/16 for subtype 'non-B'), while the FRR of the BED-CEIA was 7% (7/101 for subtype B) and 25% (4/16 for subtype 'non-B') (all p>0.05). Misclassification of ARV-treated individuals and SLP was rare by LAg (1/ 14, 0/5) and BioRad Avidity assays (2/14, 1/5) but more frequent by BED-CEIA (5/14, 3/5). Among recently-infected individuals (subtype B), 60% (15/25) were correctly classified by BED-CEIA, 88% (22/25) by BioRad Avidity and significantly fewer by LAg (48%, 12/25) compared to BioRad Avidity (p = 0.005) with a higher true-recency rate among non-B infections for all assays. Conclusions: This study using well-characterized specimens demonstrated lower FRRs for both avidity methods than with the BED-CEIA. For recently infected individuals the BioRad Avidity assay was shown to give the most accurate results.
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页数:8
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