DNA polymerase β inhibitors from Tetracera boiviniana

被引：40
作者：
Ma, J
Starck, SR
Hecht, SM
机构：
[1] Univ Virginia, Dept Chem, Charlottesville, VA 22901 USA
[2] Univ Virginia, Dept Biol, Charlottesville, VA 22901 USA
来源：
JOURNAL OF NATURAL PRODUCTS | 1999年 / 62卷 / 12期
关键词：
D O I：
10.1021/np990326p
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Bioassay-guided fractionation of an active methyl ethyl ketone extract of Tetracera boiviniana, using a sensitive assay to monitor DNA. polymerase beta inhibition, resulted in the isolation of three known triterpenoids, betulinic acid (1), 3-cis-p-coumaroyl maslinic acid (2), and 3-trans-p-coumaroyl maslinic acid (3). Compounds 1-3 inhibited DNA polymerase beta with IC50 values of 14, 15, and 4.2 mu M in the presence of bovine serum albumin (BSA) and 6.5, 7.5, and 2.0 mu M in the absence of BSA, respectively. Further, compounds 1-3 potentiated the effects of bleomycin in cultured P-388D(1) cells.
引用
收藏
页码：1660 / 1663
页数：4
相关论文
共 67 条
  • [1] EXPRESSION OF HUMAN DNA POLYMERASE-BETA IN ESCHERICHIA-COLI AND CHARACTERIZATION OF THE RECOMBINANT ENZYME
    ABBOTTS, J
    SENGUPTA, DN
    ZMUDZKA, B
    WIDEN, SG
    NOTARIO, V
    WILSON, SH
    [J]. BIOCHEMISTRY, 1988, 27 (03) : 901 - 909
  • [2] ENHANCED REPAIR OF A CISPLATIN-DAMAGED REPORTER CHLORAMPHENICOL-O-ACETYLTRANSFERASE GENE AND ALTERED ACTIVITIES OF DNA-POLYMERASES ALPHA AND BETA, AND DNA-LIGASE IN CELLS OF A HUMAN-MALIGNANT GLIOMA FOLLOWING IN-VIVO CISPLATIN THERAPY
    ALIOSMAN, F
    BERGER, MS
    RAIRKAR, A
    STEIN, DE
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, 54 (01) : 11 - 19
  • [3] DNA DAMAGE AND GROWTH-INHIBITION IN CULTURED HUMAN-CELLS BY BLEOMYCIN CONGENERS
    BERRY, DE
    CHANG, LH
    HECHT, SM
    [J]. BIOCHEMISTRY, 1985, 24 (13) : 3207 - 3214
  • [4] Overexpression of DNA polymerase β in cell results in a mutator phenotype and a decreased sensitivity to anticancer drugs
    Canitrot, Y
    Cazaux, C
    Fréchet, M
    Bouayadi, K
    Lesca, C
    Salles, B
    Hoffmann, JS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) : 12586 - 12590
  • [5] MECHANISMS OF CELLULAR-RESISTANCE TO CYTOTOXIC DRUGS AND X-IRRADIATION - KEYNOTE ADDRESS
    CARMICHAEL, J
    HICKSON, ID
    [J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 1991, 20 (02): : 197 - 202
  • [6] DNA repair: Enzymatic mechanisms and relevance to drug response
    Chaney, SG
    Sancar, A
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1996, 88 (19): : 1346 - 1360
  • [7] Inhibitors of DNA polymerase β from Schoepfia californica
    Chen, J
    Zhang, YH
    Wang, LK
    Sucheck, SJ
    Snow, AM
    Hecht, SM
    [J]. CHEMICAL COMMUNICATIONS, 1998, (24) : 2769 - 2770
  • [8] DAN S, 1980, J INDIAN CHEM SOC, V57, P760
  • [9] SEQUENCE SPECIFIC CLEAVAGE OF DNA BY ANTI-TUMOR ANTIBIOTICS NEOCARZINOSTATIN AND BLEOMYCIN
    DANDREA, AD
    HASELTINE, WA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) : 3608 - 3612
  • [10] EXPRESSION OF ACTIVE-RAT DNA POLYMERASE-BETA IN ESCHERICHIA-COLI
    DATE, T
    YAMAGUCHI, M
    HIROSE, F
    NISHIMOTO, Y
    TANIHARA, K
    MATSUKAGE, A
    [J]. BIOCHEMISTRY, 1988, 27 (08) : 2983 - 2990
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