Improvements in the detection of small ruminant lentivirus infection in the blood of sheep by PCR

被引：13
作者：
Leginagoikoa, I. [1 ]
Minguijon, E. [1 ]
Berriatua, E. [2 ]
Juste, R. A. [1 ]
机构：
[1] NEIKER Tecnalia, Anim Prod & Hlth Dept, Derio 48160, Vizcaya, Spain
[2] Univ Murcia, Sch Vet, Dept Anim Hlth, E-30100 Murcia, Spain
来源：
JOURNAL OF VIROLOGICAL METHODS | 2009年 / 156卷 / 1-2期
关键词：
Lentivirus; SRLV; Sheep; Maedi; Diagnosis; ELISA; PCR; Blood samples; POLYMERASE-CHAIN-REACTION; ARTHRITIS-ENCEPHALITIS VIRUS; MAEDI-VISNA-VIRUS; PROVIRAL DNA; GOATS; DIAGNOSIS; SEROCONVERSION; TRANSMISSION; PATHOGENESIS; MACROPHAGES;
D O I：
10.1016/j.jviromet.2008.11.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The polymerase chain reaction on blood samples has been considered a complement to serological methods for the detection of small ruminant lentiviruses (SRLV) infections in sheep and goats. This is a report on the results of a study to evaluate the use of the same blood sample for the detection of infected animals by ELISA and PCR. A comparison between the results obtained by applying PCRs targeting LTR and gag sequences on blood clot, serum and peripheral blood leucocytes was made. In addition to simplifying sampling and laboratory work, the use of blood clot samples with the gag-PCR improved remarkably the detection of infected animals. Finally, this study has shown the existence of a cell-free viremia in the serum of SRLV-infected sheep. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：145 / 149
页数：5
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