Improvements in the detection of small ruminant lentivirus infection in the blood of sheep by PCR

被引：13

作者：

Leginagoikoa, I. ^{[1
]}

Minguijon, E. ^{[1
]}

Berriatua, E. ^{[2
]}

Juste, R. A. ^{[1
]}

机构：

[1] NEIKER Tecnalia, Anim Prod & Hlth Dept, Derio 48160, Vizcaya, Spain

[2] Univ Murcia, Sch Vet, Dept Anim Hlth, E-30100 Murcia, Spain

来源：

JOURNAL OF VIROLOGICAL METHODS | 2009年 / 156卷 / 1-2期

关键词：

Lentivirus; SRLV; Sheep; Maedi; Diagnosis; ELISA; PCR; Blood samples; POLYMERASE-CHAIN-REACTION; ARTHRITIS-ENCEPHALITIS VIRUS; MAEDI-VISNA-VIRUS; PROVIRAL DNA; GOATS; DIAGNOSIS; SEROCONVERSION; TRANSMISSION; PATHOGENESIS; MACROPHAGES;

D O I：

10.1016/j.jviromet.2008.11.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The polymerase chain reaction on blood samples has been considered a complement to serological methods for the detection of small ruminant lentiviruses (SRLV) infections in sheep and goats. This is a report on the results of a study to evaluate the use of the same blood sample for the detection of infected animals by ELISA and PCR. A comparison between the results obtained by applying PCRs targeting LTR and gag sequences on blood clot, serum and peripheral blood leucocytes was made. In addition to simplifying sampling and laboratory work, the use of blood clot samples with the gag-PCR improved remarkably the detection of infected animals. Finally, this study has shown the existence of a cell-free viremia in the serum of SRLV-infected sheep. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：145 / 149

页数：5

共 34 条

[1] PCR detection of colostrum-associated Maedi-Visna virus (MVV) infection and relationship with ELISA-antibody status in lambs [J].

Alvarez, V ;

Daltabuit-Test, M ;

Arranz, J ;

Leginagoikoa, I ;

Juste, RA ;

Amorena, B ;

de Andrés, D ;

Luján, L ;

Badiola, JJ ;

Berriatua, E .

RESEARCH IN VETERINARY SCIENCE, 2006, 80 (02) :226-234

[2]

ANGELOPOULOU K, 2006, VIRUS RES

[3] Transmission and control implications of seroconversion to Maedi-Visna virus in Basque dairy-sheep flocks [J].

Berriatua, E ;

Alvarez, V ;

Extramiana, B ;

González, L ;

Daltabuit, M ;

Juste, R .

PREVENTIVE VETERINARY MEDICINE, 2003, 60 (04) :265-279

[4] THE DETECTION OF HIV-1 PROVIRAL DNA BY PCR IN CLOTTED BLOOD SPECIMENS [J].

BORISKIN, YS ;

BOOTH, JC ;

FERNANDO, S ;

CARRINGTON, D ;

EVANS, MR ;

HAY, P ;

COATES, ARM .

JOURNAL OF VIROLOGICAL METHODS, 1995, 52 (1-2) :87-94

[5] Specific detection of small ruminant lentiviral nucleic acid sequences located in the proviral long terminal repeat and leader-gag regions using real-time polymerase chain reaction [J].

Brinkhof, J. M. A. ;

Van Maanen, C. ;

Wigger, R. ;

Peterson, K. ;

Houwers, D. J. .

JOURNAL OF VIROLOGICAL METHODS, 2008, 147 (02) :338-344

[6] Current concepts in the epizootiology, diagnosis, and economic importance of ovine progressive pneumonia in North America: A review [J].

Brodie, SJ ;

de la Concha-Bermejillo, A ;

Snowder, GD ;

DeMartini, JC .

SMALL RUMINANT RESEARCH, 1998, 27 (01) :1-17

[7]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[8] Molecular biology and pathogenesis of animal lentivirus infections [J].

Clements, JE ;

Zink, MC .

CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (01) :100-+

[9]

Daltabuit-Test M., 2006, Anales de Veterinaria de Murcia, V22, P57

[10] Diagnostic tests for small ruminant lentiviruses [J].

de Andrés, D ;

Klein, D ;

Watt, NJ ;

Berriatua, E ;

Torsteinsdottir, S ;

Blacklaws, BA ;

Harkiss, GD .

VETERINARY MICROBIOLOGY, 2005, 107 (1-2) :49-62

← 1 2 3 4 →