Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2

被引：6

作者：

Gowdhaman, Dharmalingam ^{[1
]}

Jeyalakshmi, Ganesan ^{[1
]}

Sugumaran, Karuppiah ^{[1
]}

Subramanian, Nagarajan Sai ^{[1
]}

Santhosh, Ramachandran Sarojini ^{[1
]}

Ponnusami, Venkatachalam ^{[1
]}

机构：

[1] SASTRA Univ, Sch Chem & Biotechnol, Thirumalaisamudram 613401, Thanjavur, India

来源：

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI | 2014年 / 39卷 / 01期

关键词：

Paper mill effluent; Bacillus aerophilus KGJ2; xylanase; thermophilic; THERMOSTABLE XYLANASE; STABLE XYLANASE;

D O I：

10.5505/tjb.2014.92300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Objective: The objective of the present study was to isolate a potential and novel Bacillus strain from paper mill effluent for the production of an industrially important xylanase. Material and Methods: A potent xylanase producing microorganism was isolated from paper mill effluent based on zone of clearance on xylan agar medium. The strain was identified based on 16S rRNA analysis and biochemical characterization. Xylanase produced by the isolated strain was partially purified and characterized for its activity and stability. Results: The xylanase produced by this Bacillus aerophilus KGJ2 was thermophilic, shows higher activity and stability at 70 degrees C. Xylanase had activity peak at pH 4.0 and was very acid stable. Birchwood xylan and beef extract were identified as best suited carbon source and nitrogen source, respectively. Conclusion: The results confirm that Bacillus aerophilus KGJ2 produced a unique acidothermotolerant xylanase.

引用

页码：70 / 77

页数：8

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