Mitomycin C in biological fluids: Comparison of disk and cartridge solid-phase extraction and quantitation by HPLC in human plasma and ultrafiltrate

The performance of particle-loaded disks and traditional, packed extraction columns is compared. In this study, solid-phase extraction and liquid chromatographic analysis of the anticancer drug mitomycin C are duplicated using two devices that contain chemically similar Cls bonded silica. The following parameters are used to assess performance: linearity, recovery, precision, and lowest limit of detection. The extraction of mitomycin C from plasma and ultrafiltrate using particle-loaded membranes achieves better precision, improved concentration ability, lower detection limits, and greater efficiency. In addiction, elution of drug from the membrane adsorbent is efficiently accomplished using a small volume of mobile phase, eliminating analyte instability problems and the need for additional time-consuming evaporation.