Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

被引：3

作者：

Acevedo, Cristian ^{[1
]}

Stach, Matthias H. ^{[2
]}

Amtmann, Anette ^{[2
]}

Young, Manuel E. ^{[1
]}

Reyes, Juan G. ^{[3
]}

Huebner, Holger ^{[2
]}

Buchholz, Rainer ^{[2
]}

机构：

[1] Univ Tecn Federico Santa Maria, Ctr Biotechnol, Valparaiso, Chile

[2] Univ Erlangen Nurnberg, Inst Bioproc Engn, D-91052 Erlangen, Germany

[3] Pontificia Univ Catolica Valparaiso, Inst Chem, Valparaiso, Chile

来源：

ELECTRONIC JOURNAL OF BIOTECHNOLOGY | 2009年 / 12卷 / 02期

关键词：

beta-Galactosidase; delta milli pH; differential pH; IN-VIVO; SENESCENCE; CELLS; BIOMARKER; CULTURES;

D O I：

10.2225/vol12-issue2-fulltext-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The beta-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure beta-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

引用

页数：5

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