Identification of Trichoderma biocontrol isolates to clades according to ap-PCR and ITS sequence analyses

被引：12
作者：
Maymon, A
Minz, D
Barbul, O
Zveibil, A
Elad, Y
Freeman, S [1 ]
机构：
[1] Agr Res Org, Volcani Ctr, Dept Plant Pathol, IL-50250 Bet Dagan, Israel
[2] Agr Res Org, Volcani Ctr, Inst Soil Water & Environm Sci, IL-50250 Bet Dagan, Israel
来源：
PHYTOPARASITICA | 2004年 / 32卷 / 04期
关键词：
anthracnose; Fragaria x ananassa; gray mold; internal transcribed spacer (ITS) region; arbitrarily-primed polymerase chain reaction (ap-PCR); ribosomal DNA;
D O I：
10.1007/BF02979848
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A collection of Trichoderma isolates, with different biocontrol capabilities, were identified by molecular methods. Arbitrarily-primed PCR (ap-PCR) using repeat motif primers was performed on DNA from a Trichoderma spp. collection of 76 isolates, and representative isolates were further characterized into three main clades by internal transcribed spacer (ITS) sequence analysis. Consequently, a reliable phylogenetic tree was constructed containing isolates belonging to the T harzianum clade (comprising T. aureoviride, T. inhamatum, and T. virens), the T. longibrachiatum and T. saturnisporum cluster, and that including the species T. asperellum, T. atroviride, T. koningii and T. viride.
引用
收藏
页码：370 / 375
页数：6
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