Mouse adenovirus type 1 infection of macrophages

被引：20

作者：

Ashley, Shanna L.

Welton, Amanda R.

Harwood, Kirsten M.

Van Rooijen, Nico ^{[2
]}

Spindler, Katherine R. ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

[2] Vrije Univ Amsterdam, VUMC, Dept Mol Cell Biol, Fac Med, NL-1081 BT Amsterdam, Netherlands

来源：

VIROLOGY | 2009年 / 390卷 / 02期

关键词：

Macrophage; Clodronate liposome; Mouse adenovirus; Monocyte; CCR2; Chemokine (C-C) motif receptor 2; Intracellular virus staining; MEDIATED GENE-TRANSFER; ALVEOLAR MACROPHAGES; IN-VIVO; MONONUCLEAR PHAGOCYTES; DENDRITIC CELLS; VIRUS INFECTION; KUPFFER CELLS; GROWTH-FACTOR; HOST-DEFENSE; MICE LACKING;

D O I：

10.1016/j.virol.2009.05.025

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mouse adenovirus type 1 (MAV-1) causes acute and persistent infections in mice, with high levels of virus found in the brain, spinal cord and spleen in acute infections. MAV-1 infects endothelial cells throughout the mouse, and monocytes/macrophages have also been implicated as targets of the virus. Here we determined the extent and functional importance of macrophage infection by MAV-1. Bone marrow-derived macrophages expressed MAV-1 mRNAs and proteins upon ex vivo infection. Adherent peritoneal macrophages from infected mice expressed viral mRNAs and produced infectious virus. Infected chemokine (C-C motif) receptor 2 (CCR2) knockout mice, which are defective for macrophage recruitment, did not show differences in survival or MAV-1 load compared to controls. In contrast, macrophage depletion using clodronate-loaded liposomes resulted in increased virus replication in spleens of a MAV-1-resistant mouse strain, BALB/cJ. Thus macrophages serve both as targets of infection and as effectors of the host response. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：307 / 314

页数：8

共 70 条

[1] Blood clearance rates of adenovirus type 5 in mice [J].