Functional analysis of an orange-spotted grouper (Epinephelus coioides) interferon gene and characterisation of its expression in response to nodavirus infection

被引：41

作者：

Chen, Young-Mao ^{[1
,2
,3
]}

Kuo, Cham-En ^{[7
]}

Chen, Guan-Ru ^{[1
,2
]}

Kao, Yu-Ting ^{[1
,2
,3
]}

Zou, Jun ^{[6
]}

Secombes, Chris J. ^{[6
]}

Chen, Tzong-Yueh ^{[1
,2
,3
,4
,5
]}

机构：

[1] Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Inst Biotechnol, Mol Genet Lab, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Translat Ctr Marine Biotechnol, Tainan 70101, Taiwan

[3] Natl Cheng Kung Univ, Agr Biotechnol Res Ctr, Tainan 70101, Taiwan

[4] Natl Cheng Kung Univ, Univ Ctr Biosci & Biotechnol, Tainan 70101, Taiwan

[5] Natl Cheng Kung Univ, Res Ctr Ocean Environm & Technol, Tainan 70101, Taiwan

[6] Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen, Scotland

[7] Tzu Hui Inst Technol, Dept Nursing, Pingtung 92641, Taiwan

来源：

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY | 2014年 / 46卷 / 02期

关键词：

Nodavirus; Two-cysteine containing type I interferon; (2C I-IFN) gene; Antiviral protein; Mx; DOUBLE-STRANDED-RNA; ATLANTIC SALMON; I INTERFERON; BETANODAVIRUS INFECTIONS; TELEOST FISH; IFN; IDENTIFICATION; ZEBRAFISH; CELLS; MX;

D O I：

10.1016/j.dci.2014.04.004

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

We cloned and sequenced 2C I-IFN, a two-cysteine containing type I interferon (I-IFN) gene, in orange-spotted grouper (Epinephelus coioides). The cDNA has 769 base pairs, the protein has 172 amino acids, and the predicted signal peptide has 18 amino acids with two cysteines. This gene is similar to I-FNs from sea bass and other teleosts. 2C I-IFN has 5 exons and 4 introns, also similar to other teleost I-IFNs. Immunohistochemical (IHC) analysis indicated that expression is predominantly membrane-localized in healthy grouper, but has a zonal distribution in nodavirus-infected grouper. Grouper infected with nodavirus had elevated levels of 2C I-IFN at 72 h and Mx at days 6-7. Recombinant 2C I-IFN activated grouper Mx, leading to upregulated antiviral activity. The grouper Mx promoter was highly induced after treatment with recombinant 2C I-IFN. The present results suggest that expression of grouper 2C I-IFN may participate in the immunologic barrier function against nodavirus. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：117 / 128

页数：12

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