Multi-biomarker responses in the freshwater mussel Dreissena polymorpha exposed to polychlorobiphenyls and metals

被引：120

作者：

Faria, Melissa ^{[2
]}

Carrasco, Luis ^{[1
]}

Diez, Sergi ^{[1
,3
]}

Riva, Maria Carmen ^{[2
]}

Bayona, Josep Maria ^{[1
]}

Barata, Carlos ^{[1
]}

机构：

[1] CSIC, Dept Environm Chem, IDAEA, E-08028 Barcelona, Spain

[2] UPC, Lab Environm Toxicol, Terrassa 08220, Spain

[3] CSIC, Dept Environm Geol, ICTJA, E-08028 Barcelona, Spain

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2009年 / 149卷 / 03期

关键词：

Oxidative stress; Dreissena polymorpha; Methyl mercury; Mercury; PCB; Cadmium; EROD; ZEBRA MUSSEL; OXIDATIVE STRESS; DIGESTIVE GLAND; COMMON MUSSEL; POLYCHLORINATED-BIPHENYLS; QUALITY ASSESSMENT; INTEGRATED USE; HEAVY-METALS; POLLUTION; METALLOTHIONEIN;

D O I：

10.1016/j.cbpc.2008.07.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Contaminant related changes in behavioral, phase I and 11 metabolizing enzymes and pro-oxidant/antioxidant processes in the freshwater mussels Dreissena polymorpha exposed to metals and PCBs were assessed. Behavioral and biochemical responses including filtering rates, key phase 1, If and antioxidant enzymes and levels of metallothioneins, glutathione, lipid peroxidation and DNA strand breaks were determined in digestive glands of mussels after being exposed to sublethal levels of mercury chloride, methyl mercury, cadmium and Aroclor 1260 during 5 days. In 7 out of 12 responses analyzed, mussels showed significant differences across treatments. Unusual properties of measured ethoxyresorufin-O-deethylase (ERCD) activities indicated that mussels lack an inducible CYP1A enzymatic activity. Despite of using similar exposure levels, inorganic and organic mercury showed different biomarker patterns of response with methyl mercury being more bio-available and unable to induce metallothionein proteins. Mussels exposed to Cd presented higher levels of metallothioneins and an enhanced metabolism of glutathione, whereas those exposed to Aroclor showed their antioxidant glutathione peroxidase related enzyme activities inhibited. Although there was evidence for increased lipid peroxidation under exposure to inorganic and organic mercury, only mussels exposed to Aroclor had significant greater levels than those in controls. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：281 / 288

页数：8

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