Buserelin Acetate Reduces Mortality and DNA Defragmentation of Bovine Sperm Cells Exposed to Oxidative Stress

The aim of this study was to examine the effect of the GnRH analogue (buserelin acetate) on the quality of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 +/- 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0 mu g/mL successively; 2 mu g/mL; 4 mu g/mL and 8 mu g/mL buserelin acetate (Buserelin (R) aniMedica, Poland). After equilibration, the straws were frozen in liquid nitrogen vapour using a computer controlled automatic freezer. After one day, straws were thawed in a water bath at 38 degrees C for 20 sec and then were examined. Semen analysis included viability, DNA defragmentation and motility of spermatozoa were being estimated. The addition of 2 mu g / mL of the GnRH analogue significantly (P<0.05) improved the percentage of live spermatozoa compared to the control group. Samples with supplemented of 2 i 4 mu g/mL of buserelin has a significant positive influence on percentage of motile sperm, progressive motility and DNA damage. Improving the quality of frozen semen due to the use of GnRH analogue additive (buserelin acetate) for bovine semen and its low cost of use in routine semen production will allow manufacturers of insemination doses to improve the quality of their product.