Development of hypoallergenic galacto-oligosaccharides on the basis of allergen analysis

被引：22

作者：

Kaneko, Kimiyuki ^{[1
]}

Watanabe, Yoko ^{[1
]}

Kimura, Kazumasa ^{[1
]}

Matsumoto, Keisuke ^{[2
]}

Mizobuchi, Takahiro ^{[3
]}

Onoue, Masaharu ^{[1
]}

机构：

[1] Yakult Cent Inst Microbiol Res, Tokyo, Japan

[2] Yakult Pharmaceut Ind Co Ltd, Tokyo, Japan

[3] Yakult Honsha Co Ltd, Dept Dev, Tokyo, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2014年 / 78卷 / 01期

关键词：

hypoallergenic galacto-oligosaccharide; prebiotics; histamine release test; allergy; CARBOHYDRATE EPITOPES; IN-VITRO; IGE; GALACTOOLIGOSACCHARIDES; ANAPHYLAXIS; LACTOSE; ASTHMA; RATS; COMPLICATIONS; ABSORPTION;

D O I：

10.1080/09168451.2014.877819

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Galacto-oligosaccharides (GOSs) are recognized as prebiotics beneficial to human health through their abilities to modulate gut microbiota. On the other hand, it has been reported that immediate allergic reactions are caused by a GOS product (Bc-GOS) produced by treating lactose with beta-galactosidase derived from Bacillus circulans. The objective of this study was to create a safer GOS product that is less likely to cause GOS-induced allergy (GOS-AL). First, we identified two derivatives of tetrasaccharide sugar chains in Bc-GOS as the factors responsible for GOS-AL by histamine release test (HRT) using blood samples obtained from two GOS-AL patients. Through our search for non-allergic GOS, we developed a new GOS product, SK-GOS, which was produced by catalyzing lactose with beta-galactosidase derived from Sporobolomyces singularis and Kluyveromyces lactis. We regard it as a hypoallergic and safe GOS product that does not cause GOS-AL.

引用

页码：100 / 108

页数：9

共 59 条

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