Mitochondria-Targeted Fluorescent and Photoacoustic Imaging of Hydrogen Peroxide in Inflammation

被引:63
作者
Chen, Xiaoxia [1 ,2 ]
Ren, Xingxing [3 ,4 ]
Zhang, Lele [1 ,2 ]
Liu, Zhengjie [1 ,2 ]
Hai, Zijuan [1 ,2 ]
机构
[1] Anhui Univ, Inst Phys Sci, Key Lab Struct & Funct Regulat Hybrid Mat, Minist Educ, Hefei 230601, Anhui, Peoples R China
[2] Anhui Univ, Inst Informat Technol, Hefei 230601, Anhui, Peoples R China
[3] Univ Sci & Technol China, Inst Immunol, Hefei 230027, Anhui, Peoples R China
[4] Univ Sci & Technol China, Sch Basic Med Sci, Div Life Sci & Med, CAS Key Lab Innate Immun & Chron Dis, Hefei 230027, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
REACTIVE OXYGEN; PROBE; THERANOSTICS; ULTRASOUND; NANOPROBE; BIOLOGY; STRESS; H2O2;
D O I
10.1021/acs.analchem.0c03506
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Hydrogen peroxide (H2O2) is a prominent reactive oxygen species with relative stability, which makes it a potential diagnostic marker for pathological states. Excessive H2O2 in mitochondria leads to oxidative stress and inflammation. However, precisely monitoring the level of H2O2 at specific organelles (e.g., mitochondria) in vivo is still of urgent necessity. Therefore, we rationally designed a mitochondria-targeted near-infrared probe TPP-HCy-BOH for fluorescent/photoacoustic (FL/PA) dual-modal imaging of overproduced H2O2 in an inflamed mouse model. TPP-HCy-BOH had a low LOD (0.348 mu M), which is comparable to those of recently reported probes for H2O2 detection. The high kinetic rate constant (k(obs) = 4.72 x 10(-3) s(-1)) of TPP-HCy-BOH toward H2O2 is superior to recently reported H2O2 probes. Compared to control probe HCy-BOH without the mitochondrial targeting moiety, TPP-HCy-BOH successfully images exogenous or endogenous H2O2 in mitochondria with an additional 2.4-fold FL increase and 4.7-fold PA increase in HeLa cells or additional 2.1-fold FL increase and 3.3-fold PA increase in RAW 264.7 cells. In LPS-induced acute inflammation in vivo, TPP-HCy-BOH is more competent to image overproduced H2O2 with additional 1.6-fold higher sensitivity of FL in abdomen and 2.0-fold higher sensitivity of PA in liver and longer retention time of 0.5 h than HCy-BOH. We anticipate that TPP-HCy-BOH could be employed for the FL/PA dual-modal diagnosis of pathological inflammation in clinic in near future.
引用
收藏
页码:14244 / 14250
页数:7
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