Rice TUTOU1 Encodes a Suppressor of cAMP Receptor-Like Protein That Is Important for Actin Organization and Panicle Development

被引:53
作者
Bai, Jiaoteng [1 ,2 ,4 ]
Zhu, Xudong [5 ]
Wang, Qing [1 ,2 ,4 ]
Zhang, Jian [1 ,2 ]
Chen, Hongqi [5 ]
Dong, Guojun [5 ]
Zhu, Lei [6 ]
Zheng, Huakun [1 ,2 ,4 ]
Xie, Qingjun [1 ,2 ,4 ]
Nian, Jinqiang [1 ,2 ]
Chen, Fan [2 ,3 ]
Fu, Ying [6 ]
Qian, Qian [5 ]
Zuo, Jianru [1 ,2 ]
机构
[1] Chinese Acad Sci, State Key Lab Plant Genom, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[2] Chinese Acad Sci, Natl Plant Gene Res Ctr, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[3] Chinese Acad Sci, State Key Lab Mol Dev Biol, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[5] Chinese Acad Agr Sci, China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
[6] China Agr Univ, Dept Plant Sci, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China
基金
中国国家自然科学基金;
关键词
WAVE-COMPLEX SUBUNIT; II FORMIN FH5; ARP2/3; COMPLEX; POSTMEIOTIC ANTHER; CELL MORPHOGENESIS; ABC TRANSPORTER; ARPC2; SUBUNIT; ARABIDOPSIS; SCAR; WASP;
D O I
10.1104/pp.15.00229
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Panicle development, a key event in rice (Oryza sativa) reproduction and a critical determinant of grain yield, forms a branched structure containing multiple spikelets. Genetic and environmental factors can perturb panicle development, causing panicles to degenerate and producing characteristic whitish, small spikelets with severely reduced fertility and yield; however, little is known about the molecular basis of the formation of degenerating panicles in rice. Here, we report the identification and characterization of the rice panicle degenerative mutant tutou1 (tut1), which shows severe defects in panicle development. The tut1 also shows a pleiotropic phenotype, characterized by short roots, reduced plant height, and abnormal development of anthers and pollen grains. Molecular genetic studies revealed that TUT1 encodes a suppressor of cAMP receptor/Wiskott-Aldrich syndrome protein family verprolin-homologous (SCAR/WAVE)-like protein. We found that TUT1 contains conserved functional domains found in eukaryotic SCAR/WAVE proteins, and was able to activate Actin-related protein2/3 to promote actin nucleation and polymerization in vitro. Consistently, tut1 mutants show defects in the arrangement of actin filaments in trichome. These results indicate that TUT1 is a functional SCAR/WAVE protein and plays an important role in panicle development.
引用
收藏
页码:1179 / 1191
页数:13
相关论文
共 62 条
[1]   Cytokinin oxidase regulates rice grain production [J].
Ashikari, M ;
Sakakibara, H ;
Lin, SY ;
Yamamoto, T ;
Takashi, T ;
Nishimura, A ;
Angeles, ER ;
Qian, Q ;
Kitano, H ;
Matsuoka, M .
SCIENCE, 2005, 309 (5735) :741-745
[2]   DISTORTED3/SCAR2 is a putative arabidopsis WAVE complex subunit that activates the Arp2/3 complex and is required for epidermal morphogenesis [J].
Basu, D ;
Le, J ;
El-Essal, SED ;
Huang, S ;
Zhang, CH ;
Mallery, EL ;
Koliantz, G ;
Staiger, CJ ;
Szymanski, DB .
PLANT CELL, 2005, 17 (02) :502-524
[3]   Pathway of actin filament branch formation by Arp2/3 complex [J].
Beltzner, Christopher C. ;
Pollard, Thomas D. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2008, 283 (11) :7135-7144
[4]   Characterization of Arabidopsis ABCG11/WBC11, an ATP binding cassette (ABC) transporter that is required for cuticular lipid secretion [J].
Bird, David ;
Beisson, Fred ;
Brigham, Alexandra ;
Shin, John ;
Greer, Stephen ;
Jetter, Reinhard ;
Kunst, Ljerka ;
Wu, Xuemin ;
Yephremov, Alexander ;
Samuels, Lacey .
PLANT JOURNAL, 2007, 52 (03) :485-498
[5]   Direct observation of dendritic actin filament networks nucleated by Arp2/3 complex and WASP/Scar proteins [J].
Blanchoin, L ;
Amann, KJ ;
Higgs, HN ;
Marchand, JB ;
Kaiser, DA ;
Pollard, TD .
NATURE, 2000, 404 (6781) :1007-1011
[6]   Fine Mapping of qPAA8, a Gene Controlling Panicle Apical Development in Rice [J].
Cheng, Zhi-Jun ;
Mao, Bi-Gang ;
Gao, Su-Wei ;
Zhang, Ling ;
Wang, Jiu-Lin ;
Lei, Cai-Lin ;
Zhang, Xin ;
Wu, Fu-Qing ;
Guo, Xiu-Ping ;
Wan, Jianmin .
JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2011, 53 (09) :710-718
[7]   Arp2/3 and scar: Plants move to the fore [J].
Deeks, MJ ;
Hussey, PJ .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (12) :954-964
[8]   Arabidopsis NAP1 is essential for Arp2/3-dependent trichome morphogenesis [J].
Deeks, MJ ;
Kaloriti, D ;
Davies, B ;
Malhó, R ;
Hussey, PJ .
CURRENT BIOLOGY, 2004, 14 (15) :1410-1414
[9]   Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function [J].
Di Nardo, A ;
Cicchetti, G ;
Falet, H ;
Hartwig, JH ;
Stossel, TP ;
Kwiatkowski, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16263-16268
[10]   Arabidopsis GNARLED encodes a NAP125 homolog that positively regulates ARP2/3 [J].
El-Assal, SED ;
Le, J ;
Basu, D ;
Mallery, EL ;
Szymanski, DB .
CURRENT BIOLOGY, 2004, 14 (15) :1405-1409