Glucose biosensor based on polyaniline synthesized in ionic liquid

被引：15

作者：

Shi, Qiaofang ^{[1
]}

Wang, Peng ^{[1
]}

Jiang, Yan ^{[1
]}

Kan, Jinqing ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Peoples R China

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2009年 / 27卷 / 01期

基金：

美国国家科学基金会;

关键词：

Ionic liquid; intrinsic polyaniline; glucose biosensor; AC impedance; BIOELECTROCHEMICAL RESPONSES; OXIDASE ELECTRODE; SOL-GEL; POLYPYRROLE; ENZYME; FILMS; DEVICES; CONSTRUCTION; POLYMERS;

D O I：

10.1080/10242420802400025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of good electro-active polyaniline at high pH for immobilizing glucose oxidase is reported. The response current increased with increasing potential from 0.35 to 0.70 V. The maximum response current occurred at about pH 7.0. The relationship between response current and the glucose concentration was linear from 0.005 to 10.0 mmol dm(-3). The Michaelis-Menten constant K-m', maximum response current i(max) and the apparent activation energy (E-a) were 31.59 mmol dm(-3), 21.28 mu A and 32.58 kJ mol(-1), respectively. The response currents of the biosensor increased with increasing temperature. The biosensor was characterized by FTIR, UV-Vis spectra and AC impedance.

引用

页码：54 / 59

页数：6

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