Characterization of Human Cancer Cell Lines by Reverse-phase Protein Arrays

被引：160

作者：

Li, Jun ^{[1
]}

Zhao, Wei ^{[2
]}

Akbani, Rehan ^{[1
]}

Liu, Wenbin ^{[1
]}

Ju, Zhenlin ^{[1
]}

Ling, Shiyun ^{[1
]}

Vellano, Christopher P. ^{[2
]}

Roebuck, Paul ^{[1
]}

Yu, Qinghua ^{[2
]}

Eterovic, A. Karina ^{[2
]}

Byers, Lauren A. ^{[3
]}

Davies, Michael A. ^{[2
,4
]}

Deng, Wanleng ^{[4
]}

Gopal, Y. N. Vashisht ^{[4
]}

Chen, Guo ^{[4
]}

von Euw, Erika M. ^{[5
]}

Slamon, Dennis ^{[5
]}

Conklin, Dylan ^{[5
]}

Heymach, John V. ^{[3
,6
]}

Gazdar, Adi F. ^{[7
]}

Minna, John D. ^{[7
]}

Myers, Jeffrey N. ^{[8
]}

Lu, Yiling ^{[2
]}

Mills, Gordon B. ^{[2
]}

Liang, Han ^{[1
,2
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Dept Syst Biol, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Thorac Head & Neck Med Oncol, Houston, TX 77030 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Melanoma Med Oncol, Houston, TX 77030 USA

[5] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Div Hematol Oncol, Los Angeles, CA 90404 USA

[6] Univ Texas MD Anderson Canc Ctr, Dept Canc Biol, Houston, TX 77030 USA

[7] Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Therapeut Oncol Res, Dallas, TX 75390 USA

[8] Univ Texas MD Anderson Canc Ctr, Dept Head & Neck Surg, Houston, TX 77030 USA

来源：

CANCER CELL | 2017年 / 31卷 / 02期

关键词：

PROTEOGENOMIC CHARACTERIZATION; SOMATIC MUTATIONS; NETWORK ANALYSIS; RESISTANCE; SENSITIVITY; MICROARRAYS; INHIBITOR; RESOURCE; EGFR;

D O I：

10.1016/j.ccell.2017.01.005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cancer cell lines are major model systems for mechanistic investigation and drug development. However, protein expression data linked to high-quality DNA, RNA, and drug-screening data have not been available across a large number of cancer cell lines. Using reverse-phase protein arrays, we measured expression levels of similar to 230 key cancer-related proteins in >650 independent cell lines, many of which have publically available genomic, transcriptomic, and drug-screening data. Our dataset recapitulates the effects of mutated pathways on protein expression observed in patient samples, and demonstrates that proteins and particularly phosphoproteins provide information for predicting drug sensitivity that is not available from the corresponding mRNAs. We also developed a user-friendly bioinformatic resource, MCLP, to help serve the biomedical research community.

引用

页码：225 / 239

页数：15

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