Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae

被引：19

作者：

Yao, Fang-Jie ^{[1
,2
]}

Lu, Li-Xin ^{[2
]}

Wang, Peng ^{[2
]}

Fang, Ming ^{[1
]}

Zhang, You-Min ^{[1
]}

Chen, Ying ^{[2
]}

Zhang, Wei-Tong ^{[2
]}

Kong, Xiang-Hui ^{[2
]}

Lu, Jia ^{[1
]}

Honda, Yoichi ^{[3
]}

机构：

[1] Jilin Agr Univ, Coll Hort, Changchun, Jilin, Peoples R China

[2] Jilin Agr Univ, Engn Res Ctr, Chinese Minist Educ Edible & Med Fungi, Changchun, Jilin, Peoples R China

[3] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto, Japan

来源：

MYCOBIOLOGY | 2018年 / 46卷 / 01期

关键词：

Auricularia auricula-judae; fruiting body pattern; sequence-related amplified polymorphism; sequence characterized amplified region; SCAR MARKERS; RESISTANCE; MUSHROOM; DISEASE; COLOR;

D O I：

10.1080/12298093.2018.1454004

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker "SCL-18" consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster-type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.

引用

页码：72 / 78

页数：7

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