Distinct association of an alphasatellite and a betasatellite with Tomato leaf curl New Delhi virus in field-infected cucurbit

被引：14

作者：

Anwar, Sidra ^{[1
]}

机构：

[1] NUST, Atta Ur Rahman Sch Appl Biosci ASAB, H-12, Islamabad, Pakistan

来源：

JOURNAL OF GENERAL PLANT PATHOLOGY | 2017年 / 83卷 / 03期

关键词：

Bipartite; Rolling circle amplification; Yellow mosaic; DNA-BETA; MOSAIC-VIRUS; SEQUENCE; DIVERSITY; GEMINIVIRUSES; RECOMBINATION; PEPPER;

D O I：

10.1007/s10327-017-0709-8

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Leaf samples of Cucurbita pepo with yellow mosaic disease symptoms were collected in 2012. Rolling circle amplification and PCR amplification with begomovirus-specific primers confirmed the presence of an Old World bipartite begomovirus, an alphasatellite and a betasatellite. Molecular analysis of full-length sequences showed that Tomato leaf curl New Delhi virus (DNA-A) is associated with its cognate DNA-B, Papaya leaf curl betasatellite and a novel alphasatellite. To the best of our knowledge, this is the first report of an alphasatellite and a betasatellite associated with a bipartite begomovirus.

引用

页码：185 / 188

页数：4

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