microRNA-211 regulates cell proliferation, apoptosis and migration/invasion in human osteosarcoma via targeting EZRIN

被引：22

作者：

Pei, Yihua ^{[1
,2
]}

Yao, Qin ^{[1
,2
]}

Li, Yingchao ^{[3
]}

Zhang, Xin ^{[4
]}

Xie, Bozhen ^{[3
]}

机构：

[1] Xiamen Univ, ZhongShan Hosp, Cent Lab, Xiamen 361004, Fujian, Peoples R China

[2] Xiamen Univ, Affiliated ZhongShan Hosp, Fujian Prov Key Lab Chron Liver Dis & Hepatocellu, Xiamen 361004, Fujian, Peoples R China

[3] Xiamen Univ, ZhongShan Hosp, Dept Spine Surg, 201 Hubin South Rd, Xiamen 361004, Fujian, Peoples R China

[4] Xiamen Univ, ZhongShan Hosp, Dept Rehabil, Xiamen 361004, Fujian, Peoples R China

来源：

CELLULAR & MOLECULAR BIOLOGY LETTERS | 2019年 / 24卷 / 1期

基金：

中国国家自然科学基金;

关键词：

miR211; EZRIN; Osteosarcoma; Cell viability; Cell apoptosis; NEGATIVE BREAST-CANCER; MESENCHYMAL STEM-CELLS; VITAMIN-E; CARCINOMA; INVASION; GENE; METASTASIS; EXPRESSION; SURVIVAL; IGF-1;

D O I：

10.1186/s11658-019-0173-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

BackgroundIn recent years, microRNA-211 (miR211) has been considered as a tumor suppressor in multiple malignancies. However, the function of miR211 in human osteosarcoma has not been explored intensively so far. In this study, the relationship between miR211 and EZRIN was analyzed in human osteosarcoma.MethodsThe expression levels of miR211 and EZRIN were measured in both human osteosarcoma cells and tissues. The direct regulatory relationship between miR211 and EZRIN was evaluated using dual-luciferase assay. The effect of miR211 and EZRIN overexpression on cell proliferation, migration/invasion, and apoptosis was detected.ResultsThe expression of miR211 was obviously lower in osteosarcoma tissues than paracancerous tissues. EZRIN was identified as the direct target of miR211, and up-regulation of miR211 increased the percentage of cell apoptosis, and suppressed cell proliferation as well as cell migration/invasion via directly regulating EZRIN.ConclusionsOur study indicated that miR211 has an important role in the development and progress of osteosarcoma, and it might become a novel target in the diagnosis and treatment of human osteosarcoma.

引用

页数：14

共 48 条

[11] MiR-211/STAT5A Signaling Modulates Migration of Mesenchymal Stem Cells to Improve its Therapeutic Efficacy [J].

Hu, Xinyang ;

Chen, Panpan ;

Wu, Yan ;

Wang, Kan ;

Xu, Yinchuan ;

Chen, Han ;

Zhang, Ling ;

Wu, Rongrong ;

Webster, Keith A. ;

Yu, Hong ;

Zhu, Wei ;

Wang, Jian'an .

STEM CELLS, 2016, 34 (07) :1846-1858

[12] Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response [J].

Huang Ke ;

Liu PengFei ;

Li Xiang ;

Chen ShuBin ;

Wang LiHui ;

Qin Li ;

Su ZhengHui ;

Huang WenHao ;

Liu JuLi ;

Jia Bei ;

Liu Jie ;

Cai JingLei ;

Pei DuanQing ;

Pan GuangJin .

SCIENCE CHINA-LIFE SCIENCES, 2014, 57 (02) :162-170

[13] Ezrin mediates both HGF/Met autocrine and non-autocrine signaling-induced metastasis in melanoma [J].

Huang, Liping ;

Qin, Yifei ;

Zuo, Qiang ;

Bhatnagar, Kavita ;

Xiong, Jingbo ;

Merlino, Glenn ;

Yu, Yanlin .

INTERNATIONAL JOURNAL OF CANCER, 2018, 142 (08) :1652-1663

[14] Genome-wide identification of target genes for miR-204 and miR-211 identifies their proliferation stimulatory role in breast cancer cells [J].

Lee, Hyunkyung ;

Lee, Seungyeon ;

Bae, Hansol ;

Kang, Han-Sung ;

Kim, Sun Jung .

SCIENTIFIC REPORTS, 2016, 6

[15] Intronic miR-211 Assumes the Tumor Suppressive Function of Its Host Gene in Melanoma [J].

Levy, Carmit ;

Khaled, Mehdi ;

Iliopoulos, Dimitrios ;

Janas, Maja M. ;

Schubert, Steffen ;

Pinner, Sophie ;

Chen, Po-Hao ;

Li, Shuqiang ;

Fletcher, Anne L. ;

Yokoyama, Satoru ;

Scott, Kenneth L. ;

Garraway, Levi A. ;

Song, Jun S. ;

Granter, Scott R. ;

Turley, Shannon J. ;

Fisher, David E. ;

Novina, Carl D. .

MOLECULAR CELL, 2010, 40 (05) :841-849

[16] The Prognostic Role of Ezrin Immunoexpression in Osteosarcoma: A Meta-Analysis of Published Data [J].

Li, Hongtao ;

Min, Daliu ;

Zhao, Hui ;

Wang, Zhiyu ;

Qi, Weixiang ;

Zheng, Shuier ;

Tang, Lina ;

He, Aina ;

Sun, Yuanjue ;

Yao, Yang ;

Shen, Zan .

PLOS ONE, 2013, 8 (06)

[17] MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2 [J].

Li, Mao ;

Shen, Yajun ;

Wang, Qin ;

Zhou, Xuefeng .

BIOCHIMIE, 2019, 158 :224-232

[18] Expression of ezrin correlates with malignant phenotype of lung cancer, and in vitro knockdown of ezrin reverses the aggressive biological behavior of lung cancer cells [J].

Li, Qingchang ;

Gao, Hui ;

Xu, Hongtao ;

Wang, Xin ;

Pan, Yongqi ;

Hao, Fengxia ;

Qiu, Xueshan ;

Stoecker, Maggie ;

Wang, Endi ;

Wang, Enhua .

TUMOR BIOLOGY, 2012, 33 (05) :1493-1504

[19] Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells [J].

Li, Qiong ;

Wu, Mingfu ;

Wang, Hui ;

Xu, Gang ;

Zhu, Tao ;

Zhang, Yongtao ;

Liu, Ping ;

Song, Anping ;

Gang, Chen ;

Han, Zhiqiang ;

Zhou, Jianfeng ;

Meng, Li ;

Lu, Yunpin ;

Wang, Shixuan ;

Ma, Ding .

CANCER LETTERS, 2008, 261 (01) :55-63

[20] miR-204/miR-211 downregulation contributes to Candidemia-induced kidney injuries via derepression of Hmx1 expression [J].

Li, Xiao-Yue ;

Zhang, Ke ;

Jiang, Zhi-Yi ;

Cai, Li-Hua .

LIFE SCIENCES, 2014, 102 (02) :139-144

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