Comparative analysis of main aromatic acids and phthalides in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and Fo-Shou-San by a validated UHPLC-TQ-MS/MS

被引：40
作者：
Li, Weixia [1 ,2 ,3 ,4 ]
Tang, Yuping [1 ,2 ,3 ]
Qian, Yefei [1 ,2 ,3 ]
Shang, Erxin [1 ,2 ,3 ]
Wang, Linyan [1 ,2 ,3 ]
Zhang, Li [1 ,2 ,3 ]
Su, Shulan [1 ,2 ,3 ]
Duan, Jin-ao [1 ,2 ,3 ]
机构：
[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resour, Nanjing 210023, Jiangsu, Peoples R China
[2] Nanjing Univ Chinese Med, Jiangsu Key Lab High Technol Res TCM Formulae, Nanjing 210023, Jiangsu, Peoples R China
[3] Nanjing Univ Chinese Med, Natl & Local Collaborat Engn Ctr Chinese Med Reso, Nanjing 210023, Jiangsu, Peoples R China
[4] Henan Univ Tradit Chinese Med, Affiliated Hosp 1, Zhengzhou 450000, Peoples R China
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2014年 / 99卷
基金：
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词：
Fo-Shou-San; Aromatic acid; Phthalide; UHPLC-TQ-MS/MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; FLIGHT MASS-SPECTROMETRY; BIOACTIVE COMPONENTS; FERULIC ACID; QUANTIFICATION; IDENTIFICATION; QUALITY;
D O I：
10.1016/j.jpba.2014.07.007
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Fo-Shou-San (FSS) is an ancient and classic formula comprised of Angelicae Sinensis Radix (Danggui, DG) and Chuanxiong Rhizoma (Chuanxiong, CX) in a weight ratio of 3:2 with nourishing blood and dissipating blood stasis activities for the treatment of blood deficiency and blood stasis. In this study, a ultra-high-performance liquid chromatography coupled with a triple quadrupole electrospray tandem mass spectrometry (UHPLC-TQ-MS/MS) method was developed for simultaneous quantification of three aromatic acids (chlorogenic acid, caffeic acid, ferulic acid) and six phthalides (senkyunolide I, senkyunolide H, senkyunolide A, butylphthalide, ligustilide and butylidenephthalide) in DG, CX and FSS. The nine components were simultaneously determined within 10 min. The proposed method was fully validated in terms of linearity, sensitivity, precision, repeatability as well as recovery. The results showed that there were significant differences in their contents of DG and CX, and there were remarkable differences between the theorized content and observed content in FSS. The content of each component in formulae was not just the simple addition among its content in the single herbs. These research results might be helpful to illustrate the drug interactions during decocting process of herb pair according to the quantity changes of these marker compounds, which would lay foundation to further reveal the compatibility rule of the herb pair and other related formulae. (C) 2014 Elsevier B.V. All rights reserved.
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页码：45 / 50
页数：6
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