Is aceticlastic methanogen composition in full-scale anaerobic processes related to acetate utilization capacity?

被引：14

作者：

Yilmaz, Vedat ^{[1
]}

Ince-Yilmaz, Ebru ^{[2
]}

Yilmazel, Yasemin Dilsad ^{[3
]}

Duran, Metin ^{[3
]}

机构：

[1] Akdeniz Univ, Dept Environm Engn, TR-07058 Antalya, Turkey

[2] Dicle Univ, Dept Biol, Diyarbakir, Turkey

[3] Villanova Univ, Civil & Environm Engn Dept, Villanova, PA 19085 USA

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2014年 / 98卷 / 11期

关键词：

Anaerobic treatment; Acetate utilization capacity; Aceticlastic methanogenesis; Methanosaeta; Methanosarcina; REVERSE TRANSCRIPTION-PCR; BIOGAS REACTORS; METHANOTHRIX-SOEHNGENII; MICROBIAL COMMUNITIES; DIGESTION PROCESSES; GROWTH-KINETICS; SEWAGE-SLUDGE; METHANOSARCINA; OXIDATION; WASTE;

D O I：

10.1007/s00253-014-5597-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, biomass samples were obtained from six municipal and nine industrial full-scale anaerobic processes to investigate whether the aceticlastic methanogen population composition is related to acetate utilization capacity and the nature of the wastewater treated, i.e. municipal sludge or industrial wastewater. Batch serum bottle tests were used to determine the specific acetate utilization rate (AUR), and a quantitative real-time polymerase chain reaction protocol was used to enumerate the acetate-utilizing Methanosaeta and Methanosarcina populations in the biomass samples. Methanosaeta was the dominant aceticlastic methanogen in all samples, except for one industrial wastewater-treating anaerobic process. However, Methanosarcina density in industrial biomass samples was higher than the Methanosarcina density in the municipal samples. The average AUR values of municipal and industrial wastewater treatment plant biomass samples were 10.49 and 10.65 mg CH3COO-/log(aceticlastic methanogen gene copy).d, respectively. One-way ANOVA test and principle component analysis showed that the acetate utilization capacities and aceticlastic methanogen community composition did not show statistically significant correlation among the municipal digesters and industrial wastewater-treating processes investigated.

引用

页码：5217 / 5226

页数：10

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