Plasmacytoid dendritic cells develop from Ly6D+ lymphoid progenitors distinct from the myeloid lineage

被引：156

作者：

Dress, Regine J. ^{[1
]}

Dutertre, Charles-Antoine ^{[1
,2
]}

Giladi, Amir ^{[3
]}

Schlitzer, Andreas ^{[1
,4
]}

Low, Ivy ^{[1
]}

Shadan, Nurhidaya Binte ^{[1
]}

Tay, Alicia ^{[1
]}

Lum, Josephine ^{[1
]}

Kairi, Muhammad Faris Bin Mohd ^{[1
]}

Hwang, You Yi ^{[1
]}

Becht, Etienne ^{[1
]}

Cheng, Yang ^{[1
]}

Chevrier, Marion ^{[1
]}

Larbi, Anis ^{[1
]}

Newell, Evan W. ^{[1
]}

Amit, Ido ^{[3
]}

Chen, Jinmiao ^{[1
]}

Ginhoux, Florent ^{[1
,5
]}

机构：

[1] Agcy Sci Technol & Res, Singapore Immunol Network, Singapore, Singapore

[2] Duke NUS Med Sch, Program Emerging Infect Dis, Singapore, Singapore

[3] Weizmann Inst Sci, Dept Immunol, Rehovot, Israel

[4] Univ Bonn, Life & Med Sci Inst, Myeloid Cell Biol, Bonn, Germany

[5] Singhlth Translat Immunol & Inflammat Ctr, Singapore, Singapore

来源：

NATURE IMMUNOLOGY | 2019年 / 20卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

TRANSCRIPTION FACTOR E2-2; HIGH-DIMENSIONAL ANALYSIS; LANGERHANS CELLS; STEADY-STATE; RNA-SEQ; B-CELL; IDENTIFICATION; EXPRESSION; DIVERSIFICATION; MONOCYTES;

D O I：

10.1038/s41590-019-0420-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dendritic cells (DC) are currently classified as conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Through a combination of single-cell transcriptomic analysis, mass cytometry, in vivo fate mapping and in vitro clonal assays, here we show that, at the single-cell level, the priming of mouse hematopoietic progenitor cells toward the pDC lineage occurs at the common lymphoid progenitor stage, indicative of early divergence of the pDC and cDC lineages. We found the transcriptional signature of a pDC precursor stage, defined here, in the IL-7R alpha(+) common lymphoid progenitor population and identified Ly6D, IL-7R alpha, CD81 and CD2 as key markers of pDC differentiation, which distinguish pDC precursors from cDC precursors. In conclusion, pDCs developed in the bone marrow from a Ly6D(h)(i)CD2(h)(i) lymphoid progenitor cell and differentiated independently of the myeloid cDC lineage.

引用

页码：852 / +

页数：15

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