Hepatoprotective activity of date fruit extracts against dichloroacetic acid-induced liver damage in rats

被引：30

作者：

El Arem, Amira ^{[1
]}

Ghrairi, Fatma ^{[2
]}

Lahouar, Lamia ^{[1
]}

Thouri, Amira ^{[1
]}

Saafi, Emna Behija ^{[1
]}

Ayed, Amel ^{[1
]}

Zekri, Mouna ^{[1
]}

Ferjani, Hanen ^{[3
]}

Haouas, Zohra ^{[4
]}

Zakhama, Abdelfattah ^{[5
]}

Achour, Lotfi ^{[1
]}

机构：

[1] Univ Monastir, Higher Inst Biotechnol Monastir, Lab Bioresources Biol Integrat & Valorizat, Monastir 5000, Tunisia

[2] Univ Sousse, Fac Med Sousse, Biochem Lab, Sousse 4002, Tunisia

[3] Fac Med Dentaire, LRSBC, Monastir 5019, Tunisia

[4] Univ Monastir, Fac Med, Lab Histol & Cytogenet, Monastir 5019, Tunisia

[5] Serv Pathol Anat CHU F Bourguiba, Monastir, Tunisia

来源：

JOURNAL OF FUNCTIONAL FOODS | 2014年 / 9卷

关键词：

Aqueous date extract; Dichloroacetic acid; Liver markers; Antioxidant; DNA fragmentation assay; Histopathology; PHOENIX-DACTYLIFERA L; INDUCED OXIDATIVE STRESS; IN-VIVO; B6C3F1; MICE; ANTIOXIDANT; EXPOSURE; HEPATOCARCINOGENICITY; TRICHLOROACETATE; CHLORPYRIFOS; METABOLISM;

D O I：

10.1016/j.jff.2014.04.018

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The hepatoprotective activity of an aqueous date extract (ADE) against dichloroacetic acid (DCA) induced liver damage in rats was investigated. The free radical scavenging activity of ADE was evaluated using the DPPH assay. The total carbohydrate phenolic, flavonoid and condensed tannins contents of the ADE were determined. Different polyphenolic compounds, namely gallic, chlorogenic, protocatechuic, ferulic, caffeic, syringic, m-hydroxybenzoic, coumaric and phenylacetic acids, and catechin, were indentified. Oral administration of the ADE to male Wistar intoxicated with DCA at 0.5 and 2 g/l as drinking water for 2 months, demonstrated a significant protective effect by lowering the levels of hepatic marker enzymes (AST, ALT, LDH and GGT) and conjugated bilirubin, and by improving the histological architecture of the rat liver. ADE attenuated oxidative stress by decreasing the extent of hepatic TBARS (thiobarbituric acid reactive substances) formation, restoring the activities of SOD, CAT and GPx and by reducing the hepatic DNA fragmentation. This study demonstrated that ADE protects rat liver from DCA-induced injury and suggests a potential therapeutic use for ADE. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：119 / 130

页数：12

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