Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA

被引：317

作者：

Fu, Lijuan ^{[1
]}

Guerrero, Candace R. ^{[2
]}

Zhong, Na ^{[3
]}

Amato, Nicholas J. ^{[2
]}

Liu, Yunhua ^{[2
]}

Liu, Shuo ^{[1
]}

Cai, Qian ^{[1
]}

Ji, Debin ^{[2
]}

Jin, Seung-Gi ^{[4
]}

Niedernhofer, Laura J. ^{[5
]}

Pfeifer, Gerd P. ^{[4
]}

Xu, Guo-Liang ^{[3
]}

Wang, Yinsheng ^{[1
,2
]}

机构：

[1] Univ Calif Riverside, Environm Toxicol Grad Program, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

[4] City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA

[5] Scripps Florida, Dept Metab & Aging, Jupiter, FL 33458 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 33期

基金：

美国国家卫生研究院;

关键词：

5-METHYLCYTOSINE; DNA; METHYLATION; 5-FORMYLCYTOSINE; DEMETHYLATION; SUBSTRATE; 5-CARBOXYLCYTOSINE; WIDESPREAD; CONVERSION; BACTERIAL;

D O I：

10.1021/ja505305z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (similar to 1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

引用

页码：11582 / 11585

页数：4

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