Comparative Analysis of the Methanogen Diversity in Horse and Pony by Using mcrA Gene and Archaeal 16S rRNA Gene Clone Libraries

被引：27

作者：

Lwin, Khin-Ohnmar ^{[1
,2
]}

Matsui, Hiroki ^{[1
]}

机构：

[1] Mie Univ, Grad Sch Bioresources, Tsu, Mie 5148507, Japan

[2] Yangon Diagnost Lab, Livestock Breeding & Vet Dept, Yangon 11121, Myanmar

来源：

ARCHAEA-AN INTERNATIONAL MICROBIOLOGICAL JOURNAL | 2014年 / 2014卷

基金：

日本学术振兴会;

关键词：

HOKKAIDO NATIVE HORSES; EQUINE LARGE-INTESTINE; PHYLOGENETIC ANALYSIS; RUMINOCOCCUS-FLAVEFACIENS; RUMEN METHANOGENS; DAIRY-COWS; POPULATIONS; SHEEP; IDENTIFICATION; DIET;

D O I：

10.1155/2014/483574

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Comparative analysis of methanogen compositions in the feces of horse and pony was carried out by constructing the alpha-subunit of methyl coenzyme-M reductase (mcrA) gene and 16S ribosomal RNA gene (16S rRNA) clone libraries. The mcrA clone library analysis indicated that Methanomicrobiales was predominant in both horse and pony. Furthermore, most of the clones of the 16S rRNA gene library showed that Methanomicrobiales was also predominant in horse and pony, but the LIBSHUFF analysis showed that the horse and pony libraries were significantly different (P < 0.05). Most of operational taxonomic units (OTUs) showed low similarity to the identified methanogens in both the mcrA and the 16S rRNA clone libraries. The results suggest that horse and pony harbor unidentified and novel methanogens in their hindgut. The methanogen population was higher in horse than in pony; however, the anaerobic fungal population was similar in horse and pony. The methanogen diversity was different between two breeds of Equus caballus.

引用

页数：10

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