Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16

被引：49

作者：

Chong, Gang-Gang ^{[1
]}

Huang, Xiao-Jun ^{[1
]}

Di, Jun-Hua ^{[1
]}

Xu, Dao-Zhu ^{[1
]}

He, Yu-Cai ^{[1
,2
,3
]}

Pei, Ya-Nan ^{[1
]}

Tang, Ya-Jie ^{[3
]}

Ma, Cui-Luan ^{[2
]}

机构：

[1] Changzhou Univ, Coll Pharmaceut Engn & Life Sci, Biochem Engn Lab, Changzhou, Peoples R China

[2] Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Hubei Key Lab Ind Biotechnol, Wuhan, Hubei, Peoples R China

[3] Hubei Univ Technol, Hubei Prov Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Hubei Key Lab Ind Microbiol,Minist Educ, Wuhan, Hubei, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2018年 / 41卷 / 04期

关键词：

Alkali lignin; Biodegradation; High-throughput screening; Lipid; Rhodococcus pyridinivorans CCZU-B16; IN-SITU SACCHARIFICATION; LIQUID-TOLERANT CELLULASES; GALACTOMYCES SP CCZU11-1; KRAFT LIGNIN; IONIC LIQUID; LIPID PRODUCTION; DEGRADATION; PRETREATMENT; STRAINS; LIGNOCELLULOSE;

D O I：

10.1007/s00449-017-1884-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 A degrees C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future. [GRAPHICS] .

引用

页码：501 / 510

页数：10

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