Biodegradation of alkali lignin by a newly isolated Rhodococcus pyridinivorans CCZU-B16

被引：49

作者：

Chong, Gang-Gang ^{[1
]}

Huang, Xiao-Jun ^{[1
]}

Di, Jun-Hua ^{[1
]}

Xu, Dao-Zhu ^{[1
]}

He, Yu-Cai ^{[1
,2
,3
]}

Pei, Ya-Nan ^{[1
]}

Tang, Ya-Jie ^{[3
]}

Ma, Cui-Luan ^{[2
]}

机构：

[1] Changzhou Univ, Coll Pharmaceut Engn & Life Sci, Biochem Engn Lab, Changzhou, Peoples R China

[2] Hubei Univ, Hubei Collaborat Innovat Ctr Green Transformat Bi, Coll Life Sci, Hubei Key Lab Ind Biotechnol, Wuhan, Hubei, Peoples R China

[3] Hubei Univ Technol, Hubei Prov Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn, Hubei Key Lab Ind Microbiol,Minist Educ, Wuhan, Hubei, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2018年 / 41卷 / 04期

关键词：

Alkali lignin; Biodegradation; High-throughput screening; Lipid; Rhodococcus pyridinivorans CCZU-B16; IN-SITU SACCHARIFICATION; LIQUID-TOLERANT CELLULASES; GALACTOMYCES SP CCZU11-1; KRAFT LIGNIN; IONIC LIQUID; LIPID PRODUCTION; DEGRADATION; PRETREATMENT; STRAINS; LIGNOCELLULOSE;

D O I：

10.1007/s00449-017-1884-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Based on the Prussian blue spectrophotometric method, one high-throughput screening strategy for screening lignin-degrading microorganisms was built on 24-well plate at room temperature. One high activity of alkali lignin-degrading strain Rhodococcus pyridinivorans CCZU-B16 was isolated from soil. After the optimization of biodegradation, 30.2% of alkali lignin (4 g/L) was degraded under the nitrogen-limited condition (30/1 of C/N ratio; g/g) at 30 A degrees C for 72 h. It was found that syringyl (S) units and guaiacyl (G) in lignin decreased after biodegradation. Moreover, the accumulated lipid in cells had a fatty acid profile rich in C16 and C18 with four major constituent fatty acids including palmitic acid (C16:0; 22.4%), palmitoleic acid (C16:1; 21.1%), stearic acid (C18:0; 16.2%), and oleic acid (C18:1; 23.1%). In conclusion, Rhodococcus pyridinivorans CCZU-B16 showed high potential application in future. [GRAPHICS] .

引用

页码：501 / 510

页数：10

共 44 条

[1] Production of renewable gasoline from aqueous phase hydrogenation of lignin pyrolysis oil [J].

Ben, Haoxi ;

Mu, Wei ;

Deng, Yulin ;

Ragauskas, Arthur J. .

FUEL, 2013, 103 :1148-1153

[2] Pathways for degradation of lignin in bacteria and fungi [J].

Bugg, Timothy D. H. ;

Ahmad, Mark ;

Hardiman, Elizabeth M. ;

Rahmanpour, Rahman .

NATURAL PRODUCT REPORTS, 2011, 28 (12) :1883-1896

[3] Characterisation and optimisation of three potential aerobic bacterial strains for kraft lignin degradation from pulp paper waste [J].

Chandra, R. ;

Raj, A. ;

Purohit, H. J. ;

Kapley, A. .

CHEMOSPHERE, 2007, 67 (04) :839-846

[4]

Chandra R, 2013, J ENVIRON BIOL, V34, P991

[5] Isolation of Bacillus sp strains capable of decomposing alkali lignin and their application in combination with lactic acid bacteria for enhancing cellulase performance [J].

Chang, Young-Cheol ;

Choi, Dubok ;

Takamizawa, Kazuhiro ;

Kikuchi, Shintaro .

BIORESOURCE TECHNOLOGY, 2014, 152 :429-436

[6] Co-cultured Production of Lignin-Modifying Enzymes with White-Rot Fungi [J].

Chen Qi-he ;

Kruegener, Sven ;

Hirth, Thomas ;

Rupp, Steffen ;

Zibek, Susanne .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2011, 165 (02) :700-718

[7] Biodegradation of kraft lignin by a bacterial strain Comamonas sp B-9 isolated from eroded bamboo slips [J].

Chen, Y. H. ;

Chai, L. Y. ;

Zhu, Y. H. ;

Yang, Z. H. ;

Zheng, Y. ;

Zhang, H. .

JOURNAL OF APPLIED MICROBIOLOGY, 2012, 112 (05) :900-906

[8] Kraft lignin biodegradation by Novosphingobium sp B-7 and analysis of the degradation process [J].

Chen, Yuehui ;

Chai, Liyuan ;

Tang, Chongjian ;

Yang, Zhihui ;

Zheng, Yu ;

Shi, Yan ;

Zhang, Huan .

BIORESOURCE TECHNOLOGY, 2012, 123 :682-685

[9] Enhancing the enzymatic saccharification of bamboo shoot shell by sequential biological pretreatment with Galactomyces sp CCZU11-1 and deep eutectic solvent extraction [J].

Dai, Yong ;

Zhang, Hai-Sheng ;

Huan, Bin ;

He, Yucai .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2017, 40 (09) :1427-1436

[10] Value-adding to cellulosic ethanol: Lignin polymers [J].

Doherty, William O. S. ;

Mousavioun, Payam ;

Fellows, Christopher M. .

INDUSTRIAL CROPS AND PRODUCTS, 2011, 33 (02) :259-276

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