PCR Marker-Based Evaluation of the Eating Quality of Japonica Rice (Oryza sativa L.)

被引:43
作者
Lestari, Pun [1 ,2 ,6 ]
Ham, Tae-Ho [1 ,2 ]
Lee, Ho-Hoon [1 ,2 ]
Woo, Mi-Ok [1 ,2 ]
Jiang, Wenzhu [1 ,2 ]
Chu, Sang-Ho [1 ,2 ]
Kwon, Soon-Wook [4 ]
Ma, Kyungho [3 ]
Lee, Jeong-Heui [5 ]
Cho, Young-Chan [5 ]
Koh, Hee-Jong [1 ,2 ]
机构
[1] Seoul Natl Univ, Sch Plant Sci, Plant Genom & Breeding Inst, Seoul 151742, South Korea
[2] Seoul Natl Univ, Res Inst Agr & Life Sci, Seoul 151742, South Korea
[3] Rural Dev Adm, Suwon 441100, South Korea
[4] Korea Natl Open Univ, Seoul 110791, South Korea
[5] Natl Inst Crop Sci, Suwon 441100, South Korea
[6] Indonesian Ctr Agr Biotechnol & Genet Resources R, Bogor 16111, Indonesia
关键词
Rice; eating quality; palatability; marker; regression analysis; SINGLE NUCLEOTIDE POLYMORPHISMS; PHYSICOCHEMICAL PROPERTIES; VISCOSITY CHARACTERISTICS; STARCH-SYNTHASE; GENES;
D O I
10.1021/jf803804k
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Evaluation of eating quality in early breeding generations of rice is critical to developing varieties with better palatability. This paper reports DNA markers associated with eating quality of temperate japonica rice and an evaluation method aided by multiple regression analysis. A total of 30 markers comprising STSs, SNPs, and SSRs were tested for their association with palatability using 22 temperate japonica varieties with different palatability values. Eating quality-related traits of the 22 varieties were also measured. Of the 30 markers, 18 were found to be significantly associated with palatability and, consequently, a model regression equation with an R-2 value of 0.99 was formulated to estimate the palatability by the marker data set. Validation of the model equation using selected breeding lines indicated that the marker set and the equation are highly applicable to evaluation of the palatability of cooked rice in temperate japonica varieties.
引用
收藏
页码:2754 / 2762
页数:9
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