Improved Detection of Histoplasma Antigenemia following Dissociation of Immune Complexes

被引：41

作者：

Swartzentruber, S. ^{[1
]}

LeMonte, A. ^{[1
]}

Witt, J. ^{[1
]}

Fuller, D. ^{[2
]}

Davis, T. ^{[2
]}

Hage, C. ^{[2
,3
]}

Connolly, P. ^{[1
]}

Durkin, M. ^{[1
]}

Wheat, L. J. ^{[1
]}

机构：

[1] MiraVista Diagnost, Indianapolis, IN 46241 USA

[2] Indiana Univ, Dept Pathol & Lab Med, Indianapolis, IN 46204 USA

[3] Richard L Roudebush VA Med Ctr, Indianapolis, IN 46202 USA

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2009年 / 16卷 / 03期

关键词：

ENZYME-IMMUNOASSAY; AIDS;

D O I：

10.1128/CVI.00409-08

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The sensitivity for detection of Histoplasma antigen is lower in serum than in urine. In other antigen assays, treatment of serum at 104 degrees C in the presence of EDTA was required for detection of antigenemia. Sensitivity and specificity for detection of Histoplasma antigenemia were examined with or without EDTA heat treatment of the serum using the MVista Histoplasma antigen enzyme immunoassay. A total of 94.6% of serum specimens from patients with AIDS and histoplasmosis that were negative untreated were positive after EDTA-heat treatment. Two-thirds of the negative serum specimens from patients with probable histoplasmosis, based upon clinical suspicion and Histoplasma antigenuria, were positive after heat treatment. Specificity was 99.0% in controls, including healthy subjects and patients in whom histoplasmosis or blastomycosis, were excluded. Precision and reproducibility were good and excellent, respectively. These findings demonstrate improvement in sensitivity without reduction in specificity, precision, or reproducibility after heat-EDTA treatment.

引用

页码：320 / 322

页数：3

共 7 条

[1] Detection of Histoplasma antigen by a quantitative enzyme immunoassay [J].