Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3

被引：11
作者：
Wang, Huanan [1 ,2 ,7 ]
Liu, Xiangnan [3 ,4 ,5 ]
Zeng, Fanwen [3 ,4 ,5 ]
Zhang, Tongyuan [6 ]
Lian, Yuexiao [3 ,4 ,5 ]
Wu, Miaoli [3 ,4 ]
Xiao, Li [3 ,4 ]
Zhu, Yujun [3 ,4 ]
Zhang, Yu [3 ,4 ]
Chen, Meili [3 ,4 ]
Huang, Ren [3 ,4 ]
Luo, Manlin [5 ]
Cong, Feng [3 ,4 ]
Guo, Pengju [3 ,4 ]
机构：
[1] Zhejiang Univ, Zhejiang Prov Key Lab Prevent Vet Med, Dept Vet Med, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China
[3] Guangdong Lab Anim Monitoring Inst, Guangzhou 510633, Guangdong, Peoples R China
[4] Guangdong Prov Key Lab Lab Anim, Guangzhou 510633, Guangdong, Peoples R China
[5] South China Agr Univ, Coll Vet Med, Guangdong Prov Key Lab Zoonosis Prevent & Control, Guangzhou 510640, Guangdong, Peoples R China
[6] Ctr Anim Dis Control & Prevent, Fushun 113006, Peoples R China
[7] Zhejiang Univ, Coll Anim Sci, Zhejiang Prov Key Lab Prevent Vet Med, Hangzhou 310058, Zhejiang, Peoples R China
来源：
BMC VETERINARY RESEARCH | 2019年 / 15卷 / 01期
关键词：
Porcine circovirus 3; Real-time LAMP; Epidemiological investigations; PCR ASSAY; TYPE-3;
D O I：
10.1186/s12917-019-2037-z
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Background Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. Results A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 x 10(1) copies/mu L with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203). Conclusions The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.
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页数：5
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