A new strategy for protein biomarker discovery utilizing 2-nitrobenzenesulfenyl (NBS) reagent and its applications to clinical samples

被引：12
作者：
Matsuo, Ei-ichi [1 ,2 ]
Watanabe, Makoto [1 ,2 ]
Kuyama, Hiroki [1 ]
Nishimura, Osamu [1 ]
机构：
[1] Osaka Univ, Inst Prot Res, Div Dis Prote, Suita, Osaka 5650871, Japan
[2] Shimadzu Co Ltd, Technol Res Lab, Life Sci Res Ctr, Kyoto, Japan
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 25期
关键词：
Quantitative proteome; Biomarker discovery; Stable isotope; NBS method; SPECTROMETRY-BASED PROTEOMICS; MASS-SPECTROMETRY; SULFENYL HALIDES; QUANTITATIVE PROTEOMICS; GEL-ELECTROPHORESIS; TRYPTOPHAN RESIDUES; MODIFYING REAGENTS; LABELED PEPTIDES; CANCER; GENOME;
D O I：
10.1016/j.jchromb.2009.05.049
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
For the purpose of biomarker discovery, we originally developed a novel method for quantitative proteome analysis utilizing both tryptophan-targeted stable isotope tagging and mass spectrometry. The method has now been refined by replacing detergents and an enrichment column and further utilizing a novel matrix that is specifically suitable for tagged peptides. A total analytical system has been constructed by combining this method with HPLC, an automatic spotter, MALDI-TOF MS and analytical software. Clinical tissue samples such as colorectal carcinoma and renal cell carcinoma were analyzed using this system, and the results demonstrated that it is useful for discovering novel biomarker candidates. Here, we review a series of these studies and also discuss future directions for development of this technology. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：2607 / 2614
页数：8
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